The Viral Vector Core Lab will assist the affiliated projects with large scale preparation and purification of viral vectors for the program project. This entails maintaining stocks of all necessary shuttle vectors and cell lines, performing the extensive serial passaging needed for amplification of viral stocks, and purifying these viral stocks by CsCl density gradient centrifugation. Finally, the core lab will assay the purified products to determine the titers of """"""""gutted"""""""" vector and helper virus,a s well as to detect any possible contamination with replication competent adenovirus.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
5P01AG015434-03
Application #
6325714
Study Section
Project Start
2000-07-01
Project End
2001-04-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
3
Fiscal Year
2000
Total Cost
$157,631
Indirect Cost
Name
University of Michigan Ann Arbor
Department
Type
DUNS #
791277940
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
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