The overall goal of this service Core is to provide accurate and timely data to all other Projects. There are no new methodologies that need to be developed to accomplish this task. This service Core will be responsible for a set of common measurement for all Projects. Analysis of sera from Projects 1 & 2 for iron, TIBC, TSAT, and ferritin will be completed. Urine samples will be analysed for iron by atomic absorption spectrophometry using standard methods. Urine and plasma isoprostanes will be monitored as markers of oxidative stress. In the animals, hemoglobin, hematocrit, plasma iron, TIBC, TSAT, and liver non-heme iron content will be assessed for Projects 3, 4 and Core C.
Aim 1 To provide laboratory support for the determination of iron and iron-related proteins in plasma and urine and for the determination of isoprostanes, which are markers of oxidative stress, in urine and plasma from sample from human subjects.
Aim 2 : To determine the levels of tissue and brain iron in RI strains of mice.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
5P01AG021190-04
Application #
7433154
Study Section
Special Emphasis Panel (ZAG1)
Project Start
Project End
Budget Start
2007-06-01
Budget End
2008-05-31
Support Year
4
Fiscal Year
2007
Total Cost
$169,422
Indirect Cost
Name
Johns Hopkins University
Department
Type
DUNS #
001910777
City
Baltimore
State
MD
Country
United States
Zip Code
21218
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Earley, Christopher J; Kuwabara, Hiroto; Wong, Dean F et al. (2013) Increased synaptic dopamine in the putamen in restless legs syndrome. Sleep 36:51-7

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