Abstract

The individual projects in the """"""""Aging and Immunity to Infections"""""""" program require sophisticated molecular tools to analyze antigen-specific T and B cell responses, to characterize the expression of immune related genes, and to determine viral loads. The goal of the Molecular Biology Core is to support the individual projects by developing new molecular reagents and assays.
In Aims 1 and 2 the Core will develop new MHC class I and class II staining reagents for the direct identification of antigen-specific CD8+ and CD4+ T cells.
In Aim 3, the Core will develop new real-time PCR assays to quantitate mRNA expression for various viral and immune related genes, and for the quantification of viral genomes. And in Aim 4, the Core will develop approaches for the production and purification of proteins suitable for ELISAs. The Molecular Biology Core has extensive expertise in the development of these reagents and assays and preliminary data establish the feasibility of the approaches. Funds are only requested for the development of reagents and assays that are currently unavailable.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
5P01AG021600-05
Application #
7459710
Study Section
Special Emphasis Panel (ZAG1)
Project Start
2007-07-01
Project End
2008-06-30
Budget Start
2007-07-01
Budget End
2008-06-30
Support Year
5
Fiscal Year
2007
Total Cost
$87,240
Indirect Cost

  Institution

Name
Trudeau Institute, Inc.
Department
Type
DUNS #
020658969
City
Saranac Lake
State
NY
Country
United States
Zip Code
12983

  Publications

Kuchel, George A (2018) Frailty and Resilience as Outcome Measures in Clinical Trials and Geriatric Care: Are We Getting Any Closer? J Am Geriatr Soc 66:1451-1454
Lorenzo, Erica C; Bartley, Jenna M; Haynes, Laura (2018) The impact of aging on CD4+ T cell responses to influenza infection. Biogerontology 19:437-446
Parham, Kourosh; Kuchel, George A; McElhaney, Janet E et al. (2018) A Relationship Between Blood Levels of Otolin-1 and Vitamin D. Otol Neurotol 39:e269-e273
Lanzer, Kathleen G; Cookenham, Tres; Reiley, William W et al. (2018) Virtual memory cells make a major contribution to the response of aged influenza-naïve mice to influenza virus infection. Immun Ageing 15:17
Brahmakshatriya, Vinayak; Kuang, Yi; Devarajan, Priyadharshini et al. (2017) IL-6 Production by TLR-Activated APC Broadly Enhances Aged Cognate CD4 Helper and B Cell Antibody Responses In Vivo. J Immunol 198:2819-2833
Merani, Shahzma; Pawelec, Graham; Kuchel, George A et al. (2017) Impact of Aging and Cytomegalovirus on Immunological Response to Influenza Vaccination and Infection. Front Immunol 8:784
Bartley, Jenna M; Zhou, Xin; Kuchel, George A et al. (2017) Impact of Age, Caloric Restriction, and Influenza Infection on Mouse Gut Microbiome: An Exploratory Study of the Role of Age-Related Microbiome Changes on Influenza Responses. Front Immunol 8:1164
Swain, Susan L; Kugler-Umana, Olivia; Kuang, Yi et al. (2017) The properties of the unique age-associated B cell subset reveal a shift in strategy of immune response with age. Cell Immunol 321:52-60
Tabtabai, Ryan; Haynes, Laura; Kuchel, George A et al. (2017) Age-Related Increase in Blood Levels of Otolin-1 in Humans. Otol Neurotol 38:865-869
Masters, A R; Haynes, L; Su, D-M et al. (2017) Immune senescence: significance of the stromal microenvironment. Clin Exp Immunol 187:6-15

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