The Tissue Culture Core will serve all five projects in this program project. Many studies proposed inthese projects will require cultured neuronal cell lines or primary neurons or glia. The Core has considerableexperience in growing and maintaining different types of cells. Our cell culture inventory consists of over1,700 cell lines, over 130 hybridoma cell lines that produce monoclonal antibodies to apolipoproteins,lipoprotein receptors, and other proteins, and over 90 cell lines transfected with constructs that expressdifferent isoforms of apolipoprotein E, amyloid precursor protein, tau, or huntingtin or variants of thoseproteins. An online version of the inventory is available to all projects for searching and requesting cell lines.The Core will prepare the media and reagents used in culture studies (Aim 1), provide and maintain theequipment required to prepare and grow neuronal cultures (Aim 2), assist in transfection of different celllines (e.g., Neuro-2a, B103, C6, U87, HEK293T, and COS) with DMA constructs provided by investigators(Aim 3), assist in culturing primary neurons and glia (Aim 4), and help grow hybridoma cell lines formonoclonal antibody production and HEK293 cells for lentivirus production (Aim 5). The smooth operation ofthe Core and its assistance with many aspects of tissue culture studies are of fundamental importance to thesuccessful outcome of the program project.
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