Pathological analysis of nervous system tissues remains the standard for defining neurodegenerativediseases, monitoring their progression, and evaluating the effects of experimental or therapeuticmanipulations. Imaging is critical for performing neuropathological analyses and has emerged as a powerfulindependent approach for investigating dynamic physiological and pathophysiological processes. Theproposed program project will take maximal advantage of these valuable approaches. Accurate pathologicalanalyses requires the expert handling and preparation of cells and tissues for study and can require the skillsof a trained neuropathologist to interpret the findings. Effective application of imaging approaches requireswell-maintained state-of-the-art equipment, ample opportunities for education and training, and theavailability of expert consultancy. The main goals of this core are to provide the resources to project leadersof this program and enable them to address their research questions efficiently and conclusively withimaging and neuropathology approaches.To accomplish these goals, the Microscopy and Neuropathology Core proposes:
Aim 1, to assistprogram members with the fixation and processing of brain tissues and neural cultures;
Aim 2, to performroutine histological staining of cells and tissues and support immunostaining required for neuropathologicalstudies;
Aim 3, to assist and train program members in the microscopic imaging and digital documentation oftheir imaging data;
Aim 4, to provide assistance with quantitative image analysis of morphological andimmunolabeling data;
and Aim 5, to establish a tissue/section bank and maintain a corresponding onlinedatabase with Core A that is accessible to all projects.
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