Core B will be in charge of several functions supporting the 5 individual projects. Measurements of mobilization of cells in living tissue will be obtained in order to evaluate the velocity by which primitive cells migrate from the site of storage to areas of damage and promote cardiac repair. This approach will also allow us to establish under normal conditions how the activation of surface receptors on primitive cells evokes a locomotion effect and/or a growth response. The information to be obtained by two-photon microscopy will be complemented with immunocytochemical analysis of cells and myocardium by traditional confocal microscopy. In this manner, the translocated cells will be characterized in terms of expression of stem cell surface antigens, myocyte specific transcription factors, surface receptors and cytoplasmic proteins. Morphometric analysis, which is one of the functions of Core B, will be utilized to determine the absolute number of cardiac stem cells, cardiac progenitors, myocyte progenitors and myocyte precursors in various anatomical regions of the heart. Myocyte volume and number of hearts treated in different manners will be measured. Finally, this Core will provide histologic sections for all projects of the program.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
5P01AG023071-03
Application #
7260436
Study Section
Special Emphasis Panel (ZAG1)
Project Start
Project End
Budget Start
2006-07-01
Budget End
2007-06-30
Support Year
3
Fiscal Year
2006
Total Cost
$343,457
Indirect Cost
Name
New York Medical College
Department
Type
DUNS #
041907486
City
Valhalla
State
NY
Country
United States
Zip Code
10595
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Signore, Sergio; Sorrentino, Andrea; Ferreira-Martins, João et al. (2013) Inositol 1, 4, 5-trisphosphate receptors and human left ventricular myocytes. Circulation 128:1286-97
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Kajstura, Jan; Bai, Yingnan; Cappetta, Donato et al. (2012) Tracking chromatid segregation to identify human cardiac stem cells that regenerate extensively the infarcted myocardium. Circ Res 111:894-906
Anversa, Piero; Leri, Annarosa; Kajstura, Jan (2012) Biased DNA segregation during stem cell division. Circ Res 110:1403-7
Leri, Annarosa; Kajstura, Jan (2012) Created equal? The many facets of cell reprogramming. Circ Res 111:152-5

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