The major function of this core will be to prepare materials that are necessary for histological and morphometric analysis of vascular tissue from the various types of mouse models that are to be analyzed in the three projects. The major functions of the core will include preparation of sections for routine H and E staining as well as Von Geisen staining, detailed morphometric analysis, calculations of lesion area and archiving of data. The core will also be responsible for preparing tissue for immtmohistochemical staining and for the performance of immunohistochemical staining of proteins that are likely to change during vascular aging such as type I and HI collagen and elastin. The core will conduct biochemical analyses of changes in these forms of collagen and elastin. They will analyze changes in distribution of different cell types within the vascular wall such as changes in inflammatory cells that are likely to be altered in certain models that are to be analyzed. Since all of these analyses will be performed in a centralized facility using standardized methodologies it should be possible to make cross comparisons particularly among morphologic and morphometric changes that occur in the various mouse models that have been prepared. Performance of these procedures in a centralized facility is also likely to greatly facilitate the rate of progress toward completion of the specifications in these projects and will provide an objective and unbiased interpretation of the results that are obtained.
| Lozhkin, Andrey; Vendrov, Aleksandr E; Pan, Hua et al. (2017) NADPH oxidase 4 regulates vascular inflammation in aging and atherosclerosis. J Mol Cell Cardiol 102:10-21 |
| Sun, Qi-An; Runge, Marschall S; Madamanchi, Nageswara R (2016) Oxidative stress, NADPH oxidases, and arteries. Hamostaseologie 36:77-88 |
| Vendrov, Aleksandr E; Vendrov, Kimberly C; Smith, Alberto et al. (2015) NOX4 NADPH Oxidase-Dependent Mitochondrial Oxidative Stress in Aging-Associated Cardiovascular Disease. Antioxid Redox Signal 23:1389-409 |
| Madamanchi, Nageswara R; Runge, Marschall S (2013) Redox signaling in cardiovascular health and disease. Free Radic Biol Med 61:473-501 |
| Zhou, Rui-Hai; Vendrov, Aleksandr E; Tchivilev, Igor et al. (2012) Mitochondrial oxidative stress in aortic stiffening with age: the role of smooth muscle cell function. Arterioscler Thromb Vasc Biol 32:745-55 |
| Ren, Hong Yu; Patterson, Cam; Cyr, Douglas M et al. (2011) Reconstitution of CHIP E3 ubiquitin ligase activity. Methods Mol Biol 787:93-103 |
| Aitsebaomo, Julius; Srivastava, Siddharth; Zhang, Hua et al. (2011) Recombinant human interleukin-11 treatment enhances collateral vessel growth after femoral artery ligation. Arterioscler Thromb Vasc Biol 31:306-12 |
| Vendrov, Aleksandr E; Madamanchi, Nageswara R; Niu, Xi-Lin et al. (2010) NADPH oxidases regulate CD44 and hyaluronic acid expression in thrombin-treated vascular smooth muscle cells and in atherosclerosis. J Biol Chem 285:26545-57 |
| Qian, Shu-Bing; Zhang, Xingqian; Sun, Jun et al. (2010) mTORC1 links protein quality and quantity control by sensing chaperone availability. J Biol Chem 285:27385-95 |
| Monaghan-Benson, Elizabeth; Hartmann, John; Vendrov, Aleksandr E et al. (2010) The role of vascular endothelial growth factor-induced activation of NADPH oxidase in choroidal endothelial cells and choroidal neovascularization. Am J Pathol 177:2091-102 |
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