The purpose of the Otopathology/Histology Core is to provide expertise as well as coordinated and uniform assessments and experimental approaches for the histological methods common to Projects 0001, 0002 &0003.
The first aim i s to determine the otopathology (histological changes) for Projects 0001, 0002 &0003 to complement the functional assessments of hearing carried out in the Auditory Physiology Core. This will b accomplished with surface Preparations as a quantitative assessment of presence or absence of inner hair cells and outer hair cells (in all three rows) by position along the cochlear spiral. Data is viewed as a graph (cytocochleogram) and also provided as a percentage present at every location (observation point) in every cochlea. Furthermore, plastic para-modiolar sections are used for assessment of otopathology and age related changes for all cellular elements in the cochlea, including organ of Corti, auditory nerve/spiral ganglion cells and their peripheral processes, stria vascularis and lateral wall. Assessments are quantitative (Spiral ganglion cell numbers, strial width/thickness) and qualitative descriptions of pathological changes.
The second aim i s to determine the cellular localization of gene expression and proteins. Immunocytochemistry will be used for protein localization and in situ hybridization will be used to localize gene expression to specific cochlear cell types. Immunocytochemistry is performed on paramodiolar cryostat sections of the whole cochlea that allow immunostaining of all major cellular elements. Immunocytochemistry on surface preparations is used to provide additional """"""""three dimensional"""""""" resolution of immunolocalization details in the organ of Corti. In situ hybridization is used to localize gene expression to specific cochlear cell types, performed on paramodiolar cryostat sections of the whole cochlea. Data will be reported to the Data Management and evaluated and discussed with all investigators.
| Altschuler, R A; Dolan, D F; Halsey, K et al. (2015) Age-related changes in auditory nerve-inner hair cell connections, hair cell numbers, auditory brain stem response and gap detection in UM-HET4 mice. Neuroscience 292:22-33 |
| Schacht, Jochen; Altschuler, Richard; Burke, David T et al. (2012) Alleles that modulate late life hearing in genetically heterogeneous mice. Neurobiol Aging 33:1842.e15-29 |
| Gong, Tzy-Wen; Fairfield, Damon A; Fullarton, Lynne et al. (2012) Induction of heat shock proteins by hyperthermia and noise overstimulation in hsf1 -/- mice. J Assoc Res Otolaryngol 13:29-37 |
| Sha, Su-Hua; Kanicki, Ariane; Halsey, Karin et al. (2012) Antioxidant-enriched diet does not delay the progression of age-related hearing loss. Neurobiol Aging 33:1010.e15-6 |
| Miller, Richard A; Dolan, David; Han, Melissa et al. (2011) Resistance of skin fibroblasts to peroxide and UV damage predicts hearing loss in aging mice. Aging Cell 10:362-3 |
| Leiser, Scott F; Miller, Richard A (2010) Nrf2 signaling, a mechanism for cellular stress resistance in long-lived mice. Mol Cell Biol 30:871-84 |
| Sha, Su-Hua; Chen, Fu-Quan; Schacht, Jochen (2010) PTEN attenuates PIP3/Akt signaling in the cochlea of the aging CBA/J mouse. Hear Res 264:86-92 |
| Sha, Su-Hua; Chen, Fu-Quan; Schacht, Jochen (2009) Activation of cell death pathways in the inner ear of the aging CBA/J mouse. Hear Res 254:92-9 |
| Sha, Su-Hua; Kanicki, Ariane; Dootz, Gary et al. (2008) Age-related auditory pathology in the CBA/J mouse. Hear Res 243:87-94 |
| Jiang, Hongyan; Talaska, Andra E; Schacht, Jochen et al. (2007) Oxidative imbalance in the aging inner ear. Neurobiol Aging 28:1605-12 |
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