Core B - David C. Schultz, Ph. D.The Protein and Vector Production Core will be responsible for time efficient and expert technical assistancein the management of project resources, protein expression in bacteria and baculovirus infected insect cells,purification of recombinant proteins to homogeneity, and production of high-titer stocks of retroviruses (e.g.lentiviruses) for delivery of shRNA and cDNAs to mammalian cells. Expertise in all aspects of vectortechnology for protein expression, baculovirus generation, recombinant protein expression, and affinity andconventional chromatography approaches to protein purification, and production of infectious lenti-/retroviruses will be provided to all projects on a continual basis for the lifetime of grant support. The Corepersonnel are highly trained technical experts in all areas of the proposed support services to be provided tothe individual projects. The centralization and standardization of these practices under the direction of veryexperienced staff allows for high-throughput, large-volume, protein expression services, including qualityassurance and control procedures to ensure efficient, consistent production and purification of high qualityrecombinant proteins. Furthermore, confinement of retrovirus (e.g. lentivirus) production to a centralizedbiosafety level 2 (BSL2) unit provides increased biosafety, prevention of aerosolized viruses fromcontaminating incubators and parental cultures of cell lines, and quality control in the production of virusstocks to be used in experiments. In addition, the collection of projects in this proposal is expected togenerate unique research resources that will be required for programmatic interactions. The Core willfunction as a repository for collection, initial processing, storage, maintenance, and distribution of newlydeveloped materials. In this manner, resources can be reliably tracked to ensure quality control, adherenceto guidelines for resource sharing, and consistency in usage within programmatic interactions and thegreater scientific community.
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