Core - Flow Cytometry Facility

Cambier, John C.

Abstract

This core will provide complete support for the flow cytometry and cell sorting needs of program participants. This will include high rate cell sorting based on up to seven parameters (light scatter and fluorescence) and rate event analysis. Analysis of cell surface marker expression (immunofluorescence) and various static cell physiologic parameters (apoptosis, cell viability, and cell cycle) as well as real-time cell physiologic changes (calcium and magnesium mobilization, membrane potential and cytoplasmic pH changes) are available. Services will be provided using the six facility instruments that currently constitute the National Jewish Medical and Research Center institutional flow cytometry facility.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Allergy and Infectious Diseases (NIAID)
• Type: Research Program Projects (P01)
• Project #: 2P01AI022295-16
• Application #: 6412875
• Project Start: 2001-02-01
• Project End: 2002-01-31
• Support Year: 16
• Fiscal Year: 2001

Institution

• Name: National Jewish Health
• City: Denver
• State: CO
• Country: United States
• Zip Code: 80206

Publications

Ruiz-Moreno, Juan Sebastian; Hamann, Lutz; Jin, Lei et al. (2018) The cGAS/STING Pathway Detects Streptococcus pneumoniae but Appears Dispensable for Antipneumococcal Defense in Mice and Humans. Infect Immun 86:

Liu, Haolin; Wang, Chao; Lee, Schuyler et al. (2018) Specific Recognition of Arginine Methylated Histone Tails by JMJD5 and JMJD7. Sci Rep 8:3275

Getahun, Andrew; Wemlinger, Scott M; Rudra, Pratyaydipta et al. (2017) Impaired B cell function during viral infections due to PTEN-mediated inhibition of the PI3K pathway. J Exp Med 214:931-941

Liu, Haolin; Wang, Chao; Lee, Schuyler et al. (2017) Clipping of arginine-methylated histone tails by JMJD5 and JMJD7. Proc Natl Acad Sci U S A 114:E7717-E7726

Rubtsova, Kira; Rubtsov, Anatoly V; Thurman, Joshua M et al. (2017) B cells expressing the transcription factor T-bet drive lupus-like autoimmunity. J Clin Invest 127:1392-1404

Lang, Julie; Ota, Takayuki; Kelly, Margot et al. (2016) Receptor editing and genetic variability in human autoreactive B cells. J Exp Med 213:93-108

Noges, Laura E; White, Janice; Cambier, John C et al. (2016) Contamination of DNase Preparations Confounds Analysis of the Role of DNA in Alum-Adjuvanted Vaccines. J Immunol 197:1221-30

Packard, Thomas A; Smith, Mia J; Conrad, Francis J et al. (2016) B Cell Receptor Affinity for Insulin Dictates Autoantigen Acquisition and B Cell Functionality in Autoimmune Diabetes. J Clin Med 5:

Morton, J Jason; Bird, Gregory; Refaeli, Yosef et al. (2016) Humanized Mouse Xenograft Models: Narrowing the Tumor-Microenvironment Gap. Cancer Res 76:6153-6158

Getahun, Andrew; Beavers, Nicole A; Larson, Sandy R et al. (2016) Continuous inhibitory signaling by both SHP-1 and SHIP-1 pathways is required to maintain unresponsiveness of anergic B cells. J Exp Med 213:751-69