Flow cytometry/cell sorting is a critical resource for contemporary research in immunology and cell biology.All projects within this program, and Core 3, have extensive needs for this technology including, in somecases, needs for the same application and reagents. In view of this, we have integrated these needs in CoreA to accomplish economy of scale. Core A will provide for flow cytometry and cell sorting needs of programparticipants. This will include high rate cell sorting based on up to seven parameters (light scatter andfluorescence) and rare event analysis. The facility will also provide up to nine parameter analysis of cellsurface marker expression (immunofluorescence) and various static cell physiologic parameters (apoptosis,cell viability, and cell cycle), as well as real-time cell physiologic changes (calcium mobilization, intracellulartranslocation and mitochondrial membrane potential, etc.) with gating on additional parameters. Services willbe provided using the seven facility instruments that currently constitute the National Jewish Medical andResearch Center institutional flow cytometry facility.
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