The goal of this core is to provide the investigators involved in this program project, state of the art digital imaging fluorescence microscopy. This includes live cell imaging of B and T cells undergoing apoptosis;rises in intracellular Ca2+, analyzing the movement of proteins tagged with GFP or other fluorochromes;measurement of FRET;analysis of protein translocations in fixed cells;determination of the efficiency of shRNA constructs and multiparameter analysis in apoptotic cells. This core will be used by projects 2 and 3 and Core C and is essential for the successful completion of the proposed Aims described therein.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI022295-23
Application #
7929674
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
Budget Start
2009-08-01
Budget End
2010-07-31
Support Year
23
Fiscal Year
2009
Total Cost
$125,503
Indirect Cost
Name
National Jewish Health
Department
Type
DUNS #
076443019
City
Denver
State
CO
Country
United States
Zip Code
80206
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