Abstract

This project consists of five inter-related studies of B cell structure and function. Two studies address the expression of immunoglobulin genes, specifically the signals and molecular basis of isotype switching and the role of antigen structure and accessory signals needed to induce somatic variation of immunoglobulin. Projects examining non-immunoglobulin molecules in B cell function include molecular and functional studies of the cell surface glycoprotein which is the earliest marker for activated human B cells and studies designed to clone the gene for the human homolog of the mouse minor lymphocyte stimulating (Mls) antigen, a potent stimulator of T cells, and the human Fc receptor gene. both studies of human cells will pursue parallel molecular cloning strategies. Finally, B cell structures permitting or enhancing antigen presentation will be delineated and the basis for idiotype specific stimulation of T cells investigated. This program project brings together a group whose members had diverse training in virolory, molecular biology, biochemistry and cellular immunology yet share a common interest in B cell biology.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI023495-02
Application #
3091735
Study Section
(SRC)
Project Start
1986-09-30
Project End
1989-12-31
Budget Start
1988-01-01
Budget End
1988-12-31
Support Year
2
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
Tufts University
Department
Type
Schools of Medicine
DUNS #
604483045
City
Boston
State
MA
Country
United States
Zip Code
02111

  Publications

Osman, G E; Brodeur, P H; Rosenberg, N et al. (1992) The Ig VH repertoire of fetal liver-derived pre-B cells is influenced by the expression of a gene linked to X-linked immune deficiency. J Immunol 148:1928-33
Molina, I J; Huber, B T (1991) Regulation of macrophage activation markers by IL-4 and IFN-gamma is subpopulation-specific. Cell Immunol 134:241-8
Munoz, E; Zubiaga, A M; Huber, B T (1991) Tyrosine protein phosphorylation is required for protein kinase C-mediated proliferation in T cells. FEBS Lett 279:319-22
Merrow, M W; Huber, B T (1991) rIL-4 differentially regulates rIL-2-induced murine NK and LAK killing in CD8+ and CD8- precursor cell subsets. Int Immunol 3:551-61
Munoz, E; Beutner, U; Zubiaga, A et al. (1990) IL-1 activates two separate signal transduction pathways in T helper type II cells. J Immunol 144:964-9
Munoz, E; Zubiaga, A M; Merrow, M et al. (1990) Cholera toxin discriminates between T helper 1 and 2 cells in T cell receptor-mediated activation: role of cAMP in T cell proliferation. J Exp Med 172:95-103
Molina, I J; Huber, B T (1990) The expression of a tissue-specific self-peptide is required for allo-recognition. J Immunol 144:2082-8
Munoz, E; Zubiaga, A M; Munoz, J et al. (1990) Regulation of IL-4 lymphokine gene expression and cellular proliferation in murine T helper type II cells. Cell Regul 1:425-34
Kalled, S L; Brodeur, P H (1990) Preferential rearrangement of V kappa 4 gene segments in pre-B cell lines. J Exp Med 172:559-66
Molina, I J; Cannon, N A; Hyman, R et al. (1989) Macrophages and T cells do not express Mlsa determinants. J Immunol 143:39-44

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