Abstract

A core facility is operative in this laboratory for the production and maintenance monoclonal antibodies. The core is situated in a separate room equipped with laminar flow hoods, incubators, centrifuge, refrigerators, ELISA reader, and scintillation counters. The core provides the following services that are used by all program participants: 1) Assistance with the development of new monoclonal antibodies. 2) Maintenance of a large stock of cell lines and hybridomas. 3) Production of monoclonals as titred ascites and culture supernatants, purified immunoglobulin fractions, and modified Ig's (Fab fragments and biotin or fluorescein-Ig's). 4) Production of polyclonal (rabbit) antisera. 5) Mycoplasma screens and recloning of cell lines. 6) Assistance with ELISA assays and radioimmune assays.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI024775-02
Application #
3818699
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
Rockefeller University
Department
Type
DUNS #
071037113
City
New York
State
NY
Country
United States
Zip Code
10065

  Publications

Haslett, P; Hempstead, M; Seidman, C et al. (1997) The metabolic and immunologic effects of short-term thalidomide treatment of patients infected with the human immunodeficiency virus. AIDS Res Hum Retroviruses 13:1047-54
Moreira, A L; Corral, L G; Ye, W et al. (1997) Thalidomide and thalidomide analogs reduce HIV type 1 replication in human macrophages in vitro. AIDS Res Hum Retroviruses 13:857-63
Klausner, J D; Makonkawkeyoon, S; Akarasewi, P et al. (1996) The effect of thalidomide on the pathogenesis of human immunodeficiency virus type 1 and M. tuberculosis infection. J Acquir Immune Defic Syndr Hum Retrovirol 11:247-57
Bender, A; Sapp, M; Schuler, G et al. (1996) Improved methods for the generation of dendritic cells from nonproliferating progenitors in human blood. J Immunol Methods 196:121-35
Berman, M E; Xie, Y; Muller, W A (1996) Roles of platelet/endothelial cell adhesion molecule-1 (PECAM-1, CD31) in natural killer cell transendothelial migration and beta 2 integrin activation. J Immunol 156:1515-24
Frankel, S S; Wenig, B M; Burke, A P et al. (1996) Replication of HIV-1 in dendritic cell-derived syncytia at the mucosal surface of the adenoid. Science 272:115-7
Granelli-Piperno, A; Moser, B; Pope, M et al. (1996) Efficient interaction of HIV-1 with purified dendritic cells via multiple chemokine coreceptors. J Exp Med 184:2433-8
Tramontana, J M; Utaipat, U; Molloy, A et al. (1995) Thalidomide treatment reduces tumor necrosis factor alpha production and enhances weight gain in patients with pulmonary tuberculosis. Mol Med 1:384-97
Pope, M; Betjes, M G; Hirmand, H et al. (1995) Both dendritic cells and memory T lymphocytes emigrate from organ cultures of human skin and form distinctive dendritic-T-cell conjugates. J Invest Dermatol 104:11-7
Muller, W A (1995) The use of anti-PECAM reagents in the control of inflammation. Agents Actions Suppl 46:147-57

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