Core - Laboratory for Monoclonal Antibodies

Steinman, Ralph M.

Abstract

A core facility is operative in this laboratory for the production and maintenance monoclonal antibodies. The core is situated in a separate room equipped with laminar flow hoods, incubators, centrifuge, refrigerators, ELISA reader, and scintillation counters. The core provides the following services that are used by all program participants: 1) Assistance with the development of new monoclonal antibodies. 2) Maintenance of a large stock of cell lines and hybridomas. 3) Production of monoclonals as titred ascites and culture supernatants, purified immunoglobulin fractions, and modified Ig's (Fab fragments and biotin or fluorescein-Ig's). 4) Production of polyclonal (rabbit) antisera. 5) Mycoplasma screens and recloning of cell lines. 6) Assistance with ELISA assays and radioimmune assays.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Allergy and Infectious Diseases (NIAID)
• Type: Research Program Projects (P01)
• Project #: 5P01AI024775-05
• Application #: 3803521
• Support Year: 5
• Fiscal Year: 1991

Institution

• Name: Rockefeller University
• DUNS #: 071037113
• City: New York
• State: NY
• Country: United States
• Zip Code: 10065

Publications

Haslett, P; Hempstead, M; Seidman, C et al. (1997) The metabolic and immunologic effects of short-term thalidomide treatment of patients infected with the human immunodeficiency virus. AIDS Res Hum Retroviruses 13:1047-54

Moreira, A L; Corral, L G; Ye, W et al. (1997) Thalidomide and thalidomide analogs reduce HIV type 1 replication in human macrophages in vitro. AIDS Res Hum Retroviruses 13:857-63

Klausner, J D; Makonkawkeyoon, S; Akarasewi, P et al. (1996) The effect of thalidomide on the pathogenesis of human immunodeficiency virus type 1 and M. tuberculosis infection. J Acquir Immune Defic Syndr Hum Retrovirol 11:247-57

Bender, A; Sapp, M; Schuler, G et al. (1996) Improved methods for the generation of dendritic cells from nonproliferating progenitors in human blood. J Immunol Methods 196:121-35

Berman, M E; Xie, Y; Muller, W A (1996) Roles of platelet/endothelial cell adhesion molecule-1 (PECAM-1, CD31) in natural killer cell transendothelial migration and beta 2 integrin activation. J Immunol 156:1515-24

Frankel, S S; Wenig, B M; Burke, A P et al. (1996) Replication of HIV-1 in dendritic cell-derived syncytia at the mucosal surface of the adenoid. Science 272:115-7

Granelli-Piperno, A; Moser, B; Pope, M et al. (1996) Efficient interaction of HIV-1 with purified dendritic cells via multiple chemokine coreceptors. J Exp Med 184:2433-8

Berman, M E; Muller, W A (1995) Ligation of platelet/endothelial cell adhesion molecule 1 (PECAM-1/CD31) on monocytes and neutrophils increases binding capacity of leukocyte CR3 (CD11b/CD18). J Immunol 154:299-307

Granelli-Piperno, A; Pope, M; Inaba, K et al. (1995) Coexpression of NF-kappa B/Rel and Sp1 transcription factors in human immunodeficiency virus 1-induced, dendritic cell-T-cell syncytia. Proc Natl Acad Sci U S A 92:10944-8

Muller, W A (1995) The role of PECAM-1 (CD31) in leukocyte emigration: studies in vitro and in vivo. J Leukoc Biol 57:523-8