Abstract

The Laboratories of Cellular Physiology, Immunology, and Signal Transduction maintain a group of shared services, housed in separate rooms and sustained by separate skilled personnel. A proportion of each of these services is required for this program project. 1. Dishwashing and autoclave facility: In addition to these services, Lab Helpers assist in the disposal of biohazard material, i.e., all tissue culture products used for human materials, as well as pickup and delivery of mail and purchases. 2. Electron microscopy: Specimen fixation, embedding, sectioning, examination, and photography are performed by a skilled microscopist. 3. Flow cytometry: This facility, which includes FACScan and FACStar Plus instruments, is headed by an electrical engineer. He maintains all instruments, performs all of the cell sorting, coordinates computer management and data storage, and teaches all users how to employ the FACScan including data analysis. We also maintain a centralized bank of hybridomas to more than 2 dozen leukocyte antigens, for use in sorting and analytical experiments, and a central liquid nitrogen storage system.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
2P01AI024775-06
Application #
3791069
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
6
Fiscal Year
1992
Total Cost
Indirect Cost

  Institution

Name
Rockefeller University
Department
Type
DUNS #
071037113
City
New York
State
NY
Country
United States
Zip Code
10065

  Publications

Haslett, P; Hempstead, M; Seidman, C et al. (1997) The metabolic and immunologic effects of short-term thalidomide treatment of patients infected with the human immunodeficiency virus. AIDS Res Hum Retroviruses 13:1047-54
Moreira, A L; Corral, L G; Ye, W et al. (1997) Thalidomide and thalidomide analogs reduce HIV type 1 replication in human macrophages in vitro. AIDS Res Hum Retroviruses 13:857-63
Klausner, J D; Makonkawkeyoon, S; Akarasewi, P et al. (1996) The effect of thalidomide on the pathogenesis of human immunodeficiency virus type 1 and M. tuberculosis infection. J Acquir Immune Defic Syndr Hum Retrovirol 11:247-57
Bender, A; Sapp, M; Schuler, G et al. (1996) Improved methods for the generation of dendritic cells from nonproliferating progenitors in human blood. J Immunol Methods 196:121-35
Berman, M E; Xie, Y; Muller, W A (1996) Roles of platelet/endothelial cell adhesion molecule-1 (PECAM-1, CD31) in natural killer cell transendothelial migration and beta 2 integrin activation. J Immunol 156:1515-24
Frankel, S S; Wenig, B M; Burke, A P et al. (1996) Replication of HIV-1 in dendritic cell-derived syncytia at the mucosal surface of the adenoid. Science 272:115-7
Granelli-Piperno, A; Moser, B; Pope, M et al. (1996) Efficient interaction of HIV-1 with purified dendritic cells via multiple chemokine coreceptors. J Exp Med 184:2433-8
Tramontana, J M; Utaipat, U; Molloy, A et al. (1995) Thalidomide treatment reduces tumor necrosis factor alpha production and enhances weight gain in patients with pulmonary tuberculosis. Mol Med 1:384-97
Pope, M; Betjes, M G; Hirmand, H et al. (1995) Both dendritic cells and memory T lymphocytes emigrate from organ cultures of human skin and form distinctive dendritic-T-cell conjugates. J Invest Dermatol 104:11-7
Muller, W A (1995) The use of anti-PECAM reagents in the control of inflammation. Agents Actions Suppl 46:147-57

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