Core - DNA and Cell Bank

Nepom, Gerald T.

Abstract

The DNA and Cell Bank core will prepare, store, and distribute cells, DNA, and synthetic oligonucleotides to the program project investigators. The Core will also coordinate access to a central data bank with clinical and laboratory data shared between each project investigator on the VMRC immunology program computer network. Detritylated oligonucleotides (12-15/mo) will be synthesized for use as primers and probes for projects 1-4. Genomic DNA will be prepared from PBL and LCL for use in the studies in projects 3 and 4. The Cell Bank will maintain LCL and monoclonal antibody hybridoma cell lines for use by all four projects and will maintain frozen stocks of PBL or T-cell lines and clones for use in projects 1 and 2.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Allergy and Infectious Diseases (NIAID)
• Type: Research Program Projects (P01)
• Project #: 5P01AI031241-02
• Application #: 3791625
• Support Year: 2
• Fiscal Year: 1992

Institution

• Name: Benaroya Research Institute at Virginia Mason
• City: Seattle
• State: WA
• Country: United States
• Zip Code: 98101

Publications

Wei, S; Charmley, P; Concannon, P (1997) Organization, polymorphism, and expression of the human T-cell receptor AV1 subfamily. Immunogenetics 45:405-12

Brawley, J V; Concannon, P (1996) Modulation of promiscuous T cell receptor recognition by mutagenesis of CDR2 residues. J Exp Med 183:2043-51

Pinchuk, G V; Nottenburg, C; Milner, E C (1995) Predominant V-region gene configurations in the human antibody response to Haemophilus influenzae capsule polysaccharide. Scand J Immunol 41:324-30

Kovats, S; Nepom, G T; Coleman, M et al. (1995) Deficient antigen-presenting cell function in multiple genetic complementation groups of type II bare lymphocyte syndrome. J Clin Invest 96:217-23

Milner, E C; Hufnagle, W O; Glas, A M et al. (1995) Polymorphism and utilization of human VH Genes. Ann N Y Acad Sci 764:50-61

Pinchuk, G V; Nottenburg, C; Milner, E C (1995) Patterns of V-region gene segment association in the human antibody response to Haemophilus influenzae type B capsular polysaccharide. Ann N Y Acad Sci 764:378-80

Glas, A M; Hufnagle, W O; Suzuki, I et al. (1995) Anomalous diversification of the antibody repertoire following bone marrow transplantation. Ann N Y Acad Sci 764:312-4

Suzuki, I; Pfister, L; Glas, A et al. (1995) Representation of rearranged VH gene segments in the human adult antibody repertoire. J Immunol 154:3902-11

Hufnagle, W O; Huang, S C; Suzuki, I et al. (1995) A complete preimmune human VH3 repertoire. Ann N Y Acad Sci 764:293-5

Kovats, S; Drover, S; Marshall, W H et al. (1994) Coordinate defects in human histocompatibility leukocyte antigen class II expression and antigen presentation in bare lymphocyte syndrome. J Exp Med 179:2017-22