Abstract

This Clinical Laboratory Core will provide an efficient centralized laboratory for routine handling and analyses of clinical specimens in support of Projects 1 and 2. The specific functions of the Core Laboratory are: 1. To process blood and fluid samples collected in the clinical studies (Project 1) for utilization in Projects 1 and 2 and this Core, 2. To perform diagnostic serologic and virologic assays for Projects 1 and 2, 3. To quantify the levels of dengue virus RNA in plasma/serum samples by fluorogenic RT-PCR for Projects 1 and 2, and 4. To perform flow cytometry analyses on fresh blood samples for Projects 1 and 2. The Core Laboratory will be located in facilities of the Department of Virology, Armed Forces Research Institute of Medical Sciences in Bangkok and Kamphaeng Phet, Thailand. The Department has over 40 years of experience in flavivirology and clinical studies.

Public Health Relevance

A Core Clinical Laboratory will operate in Thailand to process the large number of clinical specimens collected in this research program and will perform routine laboratory tests for Projects 1 and 2. This organization provides an efficient system for routine procedures that support our research.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI034533-20
Application #
8377860
Study Section
Special Emphasis Panel (ZAI1-MMT-M)
Project Start
Project End
2013-07-17
Budget Start
2012-04-01
Budget End
2013-03-31
Support Year
20
Fiscal Year
2012
Total Cost
$708,838
Indirect Cost
$66,106

  Institution

Name
University of Rhode Island
Department
Type
DUNS #
144017188
City
Kingston
State
RI
Country
United States
Zip Code
02881

  Publications

Park, Sangshin; Srikiatkhachorn, Anon; Kalayanarooj, Siripen et al. (2018) Use of structural equation models to predict dengue illness phenotype. PLoS Negl Trop Dis 12:e0006799
Salje, Henrik; Cummings, Derek A T; Rodriguez-Barraquer, Isabel et al. (2018) Reconstruction of antibody dynamics and infection histories to evaluate dengue risk. Nature 557:719-723
Kang, Jeon-Young; Aldstadt, Jared (2017) The Influence of Spatial Configuration of Residential Area and Vector Populations on Dengue Incidence Patterns in an Individual-Level Transmission Model. Int J Environ Res Public Health 14:
Srikiatkhachorn, Anon; Mathew, Anuja; Rothman, Alan L (2017) Immune-mediated cytokine storm and its role in severe dengue. Semin Immunopathol 39:563-574
Rattanamahaphoom, Jittraporn; Leaungwutiwong, Pornsawan; Limkittikul, Kriengsak et al. (2017) Activation of dengue virus-specific T cells modulates vascular endothelial growth factor receptor 2 expression. Asian Pac J Allergy Immunol 35:171-178
Kalayanarooj, Siripen; Rothman, Alan L; Srikiatkhachorn, Anon (2017) Case Management of Dengue: Lessons Learned. J Infect Dis 215:S79-S88
Townsley, E; O'Connor, G; Cosgrove, C et al. (2016) Interaction of a dengue virus NS1-derived peptide with the inhibitory receptor KIR3DL1 on natural killer cells. Clin Exp Immunol 183:419-30
Clapham, Hannah E; Rodriguez-Barraquer, Isabel; Azman, Andrew S et al. (2016) Dengue Virus (DENV) Neutralizing Antibody Kinetics in Children After Symptomatic Primary and Postprimary DENV Infection. J Infect Dis 213:1428-35
Woda, Marcia; Friberg, Heather; Currier, Jeffrey R et al. (2016) Dynamics of Dengue Virus (DENV)-Specific B Cells in the Response to DENV Serotype 1 Infections, Using Flow Cytometry With Labeled Virions. J Infect Dis 214:1001-9
Nisalak, Ananda; Clapham, Hannah E; Kalayanarooj, Siripen et al. (2016) Forty Years of Dengue Surveillance at a Tertiary Pediatric Hospital in Bangkok, Thailand, 1973-2012. Am J Trop Med Hyg 94:1342-7

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