Abstract

Core C will provide investigators of this program project with convenient access to synthetic peptides that are economically produced. Core C provides the instrumentation and expertise for design, synthesis, quality control, and use of synthetic peptides in a shared instrumentation setting. Synthetic peptides are prepared by tBOC and Fmoc chemistries and surplus is reserved for use by other Center investigators. Peptides will be used for mapping monoclonal antibodies, preparing antibodies and identifying peptides derived from chlamydial major outer membrane protein that are presented MHC class II molecules.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
1P01AI034617-05
Application #
6235156
Study Section
Project Start
1997-09-01
Project End
2000-12-31
Budget Start
1996-10-01
Budget End
1997-09-30
Support Year
5
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
University of Wisconsin Madison
Department
Type
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715

  Publications

Kalayoglu, Murat V; Libby, Peter; Byrne, Gerald I (2002) Chlamydia pneumoniae as an emerging risk factor in cardiovascular disease. JAMA 288:2724-31
Kalayoglu, M V; Perkins, B N; Byrne, G I (2001) Chlamydia pneumoniae-infected monocytes exhibit increased adherence to human aortic endothelial cells. Microbes Infect 3:963-9
Kim, S K; DeMars, R (2001) Epitope clusters in the major outer membrane protein of Chlamydia trachomatis. Curr Opin Immunol 13:429-36
Kim, S K; Devine, L; Angevine, M et al. (2000) Direct detection and magnetic isolation of Chlamydia trachomatis major outer membrane protein-specific CD8+ CTLs with HLA class I tetramers. J Immunol 165:7285-92
Ortiz, L; Angevine, M; Kim, S K et al. (2000) T-cell epitopes in variable segments of Chlamydia trachomatis major outer membrane protein elicit serovar-specific immune responses in infected humans. Infect Immun 68:1719-23
LaVerda, D; Kalayoglu, M V; Byrne, G I (1999) Chlamydial heat shock proteins and disease pathology: new paradigms for old problems? Infect Dis Obstet Gynecol 7:64-71
Kim, S K; Angevine, M; Demick, K et al. (1999) Induction of HLA class I-restricted CD8+ CTLs specific for the major outer membrane protein of Chlamydia trachomatis in human genital tract infections. J Immunol 162:6855-66
Kane, C D; Vena, R M; Ouellette, S P et al. (1999) Intracellular tryptophan pool sizes may account for differences in gamma interferon-mediated inhibition and persistence of chlamydial growth in polarized and nonpolarized cells. Infect Immun 67:1666-71
Kalayoglu, M V; Byrne, G I (1998) A Chlamydia pneumoniae component that induces macrophage foam cell formation is chlamydial lipopolysaccharide. Infect Immun 66:5067-72
Kalayoglu, M V; Byrne, G I (1998) Induction of macrophage foam cell formation by Chlamydia pneumoniae. J Infect Dis 177:725-9

Showing the most recent 10 out of 19 publications