Abstract

Core is responsible for preparing the various peptides. Protegrins will generally be synthesized by Dr. Waring at the UCLA site. LL-37 and related alpha-helical peptides will be synthesized by Dr. Pohl at Emory, as will any protegrins that would be benefit from t-boc synthesis. The availability of high-quality peptides is absolutely essential for the proposed studies. Dr. Waring and Pohl are highly accomplished investigators with access to state-of-the-art tools for peptide synthesis, purification and characterization.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
2P01AI037945-05
Application #
6257821
Study Section
Special Emphasis Panel (ZAI1-ALR-M (M4))
Project Start
1995-03-01
Project End
2003-08-31
Budget Start
Budget End
Support Year
5
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
University of California Los Angeles
Department
Type
DUNS #
119132785
City
Los Angeles
State
CA
Country
United States
Zip Code
90095

  Publications

Gryllos, Ioannis; Tran-Winkler, Hien J; Cheng, Ming-Fang et al. (2008) Induction of group A Streptococcus virulence by a human antimicrobial peptide. Proc Natl Acad Sci U S A 105:16755-60
Doherty, Tim; Waring, Alan J; Hong, Mei (2008) Dynamic structure of disulfide-removed linear analogs of tachyplesin-I in the lipid bilayer from solid-state NMR. Biochemistry 47:1105-16
Tang, Ming; Waring, Alan J; Hong, Mei (2007) Phosphate-mediated arginine insertion into lipid membranes and pore formation by a cationic membrane peptide from solid-state NMR. J Am Chem Soc 129:11438-46
Mani, Rajeswari; Waring, Alan J; Hong, Mei (2007) Conformation, dynamics, and insertion of a noncysteine-containing protegrin-1 analogue in lipid membranes from solid-state NMR spectroscopy. Chembiochem 8:1877-84
Cole, Amy L; Yang, Otto O; Warren, Andrew D et al. (2006) HIV-1 adapts to a retrocyclin with cationic amino acid substitutions that reduce fusion efficiency of gp41. J Immunol 176:6900-5
Doherty, Tim; Waring, Alan J; Hong, M (2006) Membrane-bound conformation and topology of the antimicrobial peptide tachyplesin I by solid-state NMR. Biochemistry 45:13323-30
Mani, Rajeswari; Cady, Sarah D; Tang, Ming et al. (2006) Membrane-dependent oligomeric structure and pore formation of a beta-hairpin antimicrobial peptide in lipid bilayers from solid-state NMR. Proc Natl Acad Sci U S A 103:16242-7
Valore, Erika V; Wiley, Dorothy J; Ganz, Tomas (2006) Reversible deficiency of antimicrobial polypeptides in bacterial vaginosis. Infect Immun 74:5693-702
Doherty, Timothy; Waring, Alan J; Hong, Mei (2006) Peptide-lipid interactions of the beta-hairpin antimicrobial peptide tachyplesin and its linear derivatives from solid-state NMR. Biochim Biophys Acta 1758:1285-91
Mani, R; Tang, M; Wu, X et al. (2006) Membrane-bound dimer structure of a beta-hairpin antimicrobial peptide from rotational-echo double-resonance solid-state NMR. Biochemistry 45:8341-9

Showing the most recent 10 out of 62 publications