Abstract

This core will supply large-scale amounts of eukaryotic and prokaryotic expression system-produced proteins. Specifically, mini-proteins will be made in T7-based E. coli expression systems as inclusion bodies. In addition, Lec3.2.8.1-generated ADA, 89.6P and clades C, A and O derivatives of HIV gp140, gp120 and gp140 mini-proteins will be produced. As required, selenomethionine labeling of eukaryotically or E. coli expressed proteins will be undertaken for x-ray crystallography as well as 15N and 13C labeling of E. coli produced proteins for NMR analysis. Large-scale production of various mini-protein constructs will also be conducted. All monoclonal antibody generation, Fab antibody purification and crystallization trials will be established herein.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
2P01AI043649-05
Application #
6569538
Study Section
Special Emphasis Panel (ZRG1)
Project Start
2002-08-01
Project End
2007-07-31
Budget Start
Budget End
Support Year
5
Fiscal Year
2002
Total Cost
Indirect Cost

  Institution

Name
Dana-Farber Cancer Institute
Department
Type
DUNS #
149617367
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Tomaras, Georgia D; Montefiori, David C (2014) Spectrum of HIV antibodies in vaccine and disease. Curr Opin HIV AIDS 9:207-9
Hatfield, Stephen; Belikoff, Bryan; Lukashev, Dmitriy et al. (2009) The antihypoxia-adenosinergic pathogenesis as a result of collateral damage by overactive immune cells. J Leukoc Biol 86:545-8
Song, Likai; Sun, Zhen-Yu J; Coleman, Kate E et al. (2009) Broadly neutralizing anti-HIV-1 antibodies disrupt a hinge-related function of gp41 at the membrane interface. Proc Natl Acad Sci U S A 106:9057-62
Sun, Zhen-Yu J; Oh, Kyoung Joon; Kim, Mikyung et al. (2008) HIV-1 broadly neutralizing antibody extracts its epitope from a kinked gp41 ectodomain region on the viral membrane. Immunity 28:52-63
Kim, Mikyung; Qiao, Zhisong; Yu, Jessica et al. (2007) Immunogenicity of recombinant human immunodeficiency virus type 1-like particles expressing gp41 derivatives in a pre-fusion state. Vaccine 25:5102-14
Qiao, Zhi-Song; Kim, Mikyung; Reinhold, Bruce et al. (2005) Design, expression, and immunogenicity of a soluble HIV trimeric envelope fragment adopting a prefusion gp41 configuration. J Biol Chem 280:23138-46
Chen, Bing; Vogan, Erik M; Gong, Haiyun et al. (2005) Determining the structure of an unliganded and fully glycosylated SIV gp120 envelope glycoprotein. Structure 13:197-211
Kim, Mikyung; Qiao, Zhi-Song; Montefiori, David C et al. (2005) Comparison of HIV Type 1 ADA gp120 monomers versus gp140 trimers as immunogens for the induction of neutralizing antibodies. AIDS Res Hum Retroviruses 21:58-67
Reche, Pedro A; Zhang, Hong; Glutting, John-Paul et al. (2005) EPIMHC: a curated database of MHC-binding peptides for customized computational vaccinology. Bioinformatics 21:2140-1
Chen, Bing; Cheng, Yifan; Calder, Lesley et al. (2004) A chimeric protein of simian immunodeficiency virus envelope glycoprotein gp140 and Escherichia coli aspartate transcarbamoylase. J Virol 78:4508-16

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