Abstract

The simian core will be responsible for all monkey manipulations and the SIV virology. As such, it provides the necessary reagents required for the accomplishment of the aims of all the projects in this proposal. This core will obtain monkeys, infect them with a titered stock of HIV/DeltaB670, administered treatment with PMPA, and immunize with the vaccines provided by projects 1 and 2 according to the proposed schedule. Monkeys will be followed for signals of immunodeficiency by clinical examination, enumeration of T cell subsets, and quantitation of plasma virus loads. The results of these assays will be organized in a computer database using Blacksmith software and a Sun sparkstation networked to the project laboratories for ready access. This core will provide for the clinical care of monkeys on this project and will be responsible for the collection and distribution of blood, lymph node, and bone marrow samples to the other projects as needed. Monkeys in end-stage disease or at the termination of the experiment will be humanely sacrificed and tissues collected and distributed to project laboratories for analysis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI043664-03
Application #
6347243
Study Section
Project Start
2000-09-01
Project End
2001-08-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
3
Fiscal Year
2000
Total Cost
$213,937
Indirect Cost

  Institution

Name
University of Pittsburgh
Department
Type
DUNS #
053785812
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213

  Publications

Macatangay, Bernard J C; Riddler, Sharon A; Wheeler, Nicole D et al. (2016) Therapeutic Vaccination With Dendritic Cells Loaded With Autologous HIV Type 1-Infected Apoptotic Cells. J Infect Dis 213:1400-9
Soloff, Adam C; Liu, Xiangdong; Gao, Wentao et al. (2009) Adenovirus 5- and 35-based immunotherapy enhances the strength but not breadth or quality of immunity during chronic SIV infection. Eur J Immunol 39:2437-49
Brown, Kevin N; Trichel, Anita; Barratt-Boyes, Simon M (2007) Parallel loss of myeloid and plasmacytoid dendritic cells from blood and lymphoid tissue in simian AIDS. J Immunol 178:6958-67
Barratt-Boyes, Simon M; Soloff, Adam C; Gao, Wentao et al. (2006) Broad cellular immunity with robust memory responses to simian immunodeficiency virus following serial vaccination with adenovirus 5- and 35-based vectors. J Gen Virol 87:139-49
Gao, Wentao; Rzewski, Alexis; Sun, Huijie et al. (2004) UpGene: Application of a web-based DNA codon optimization algorithm. Biotechnol Prog 20:443-8
Barron, Michelle A; Blyveis, Naomi; Palmer, Brent E et al. (2003) Influence of plasma viremia on defects in number and immunophenotype of blood dendritic cell subsets in human immunodeficiency virus 1-infected individuals. J Infect Dis 187:26-37
Riddler, Sharon A; Smit, Ellen; Cole, Stephen R et al. (2003) Impact of HIV infection and HAART on serum lipids in men. JAMA 289:2978-82
Donnenberg, Vera S; Donnenberg, Albert D (2003) Identification, rare-event detection and analysis of dendritic cell subsets in broncho-alveolar lavage fluid and peripheral blood by flow cytometry. Front Biosci 8:s1175-80
Harshyne, Larry A; Zimmer, Michael I; Watkins, Simon C et al. (2003) A role for class A scavenger receptor in dendritic cell nibbling from live cells. J Immunol 170:2302-9
Meidenbauer, Norbert; Hoffmann, Thomas K; Donnenberg, Albert D (2003) Direct visualization of antigen-specific T cells using peptide-MHC-class I tetrameric complexes. Methods 31:160-71

Showing the most recent 10 out of 19 publications