The Virology Laboratory will serve as the central AIDS laboratory for confirmatory testing of antiviral activity of ribozyme constructs of vector- transduced cells developed in Projects 2 and 3, and will perform the HIV- monitoring and specimen processing in the gene therapy trials of Project 4. The development and screening tests for new anti-HIV genetic expression vectors is performed by Projects 2 and 3, and Core C will assist with assays (e.g., RT and p24 assays) which facilitate this research. At the appropriate phase of this development, this Core will provide the confirmatory anti-HIV challenge experiments using multiple-strains of HIV-1. For the clinical studies, this Core will provide services for granulocyte and mononuclear fractionation of blood and marrow specimens, and will coordinate the further analyses of these specimens within the IPCP program. Importance to Overall Program. This Core provides essential quality control to the scientific and clinical projects. In addition, in providing p24 and reverse transcriptase services to the scientific projects, this laboratory increase the productivity of the scientific projects. Similarly, the laboratory provides the necessary specimen processing and evaluation of blood and bone marrow for the gene therapy trials. This core serves to increased the productivity of both scientific projects and clinical activity.
| Saetrom, Pål; Biesinger, Jacob; Li, Sierra M et al. (2009) A risk variant in an miR-125b binding site in BMPR1B is associated with breast cancer pathogenesis. Cancer Res 69:7459-65 |
| Kowolik, Claudia M; Yam, Priscilla; Yu, Ying et al. (2003) HIV vector production mediated by Rev protein transduction. Mol Ther 8:324-31 |
| Li, Ming-Jie; Bauer, Gerhard; Michienzi, Alessandro et al. (2003) Inhibition of HIV-1 infection by lentiviral vectors expressing Pol III-promoted anti-HIV RNAs. Mol Ther 8:196-206 |
| Paul, Cynthia P; Good, Paul D; Li, Shirley X L et al. (2003) Localized expression of small RNA inhibitors in human cells. Mol Ther 7:237-47 |
| Strayer, David; Branco, Francisco; Zern, Mark A et al. (2002) Durability of transgene expression and vector integration: recombinant SV40-derived gene therapy vectors. Mol Ther 6:227-37 |
| Lee, Nan Sook; Dohjima, Taikoh; Bauer, Gerhard et al. (2002) Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells. Nat Biotechnol 20:500-5 |
| Yam, Priscilla Y; Li, Shulian; Wu, Jerry et al. (2002) Design of HIV vectors for efficient gene delivery into human hematopoietic cells. Mol Ther 5:479-84 |
| Michienzi, Alessandro; Li, Shirley; Zaia, John A et al. (2002) A nucleolar TAR decoy inhibitor of HIV-1 replication. Proc Natl Acad Sci U S A 99:14047-52 |
| Chang, Zongli; Westaway, Shawn; Li, Shirley et al. (2002) Enhanced expression and HIV-1 inhibition of chimeric tRNA(Lys3)-ribozymes under dual U6 snRNA and tRNA promoters. Mol Ther 6:481-9 |
| Michienzi, A; Rossi, J J (2001) Intracellular applications of ribozymes. Methods Enzymol 341:581-96 |
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