The overall goal of this program project grant is to study the mucosal barrier to infectious agents. This Core provides a facility for Live Cell Multiphoton and Confocal Imaging. Mucosal surfaces are largely lined by a monolayer of epithelial cells. These cells are highly polarized, with separate apical and basolateral surfaces. These cells are typically 10-15 microns tall. It is therefore essential to study these cells using an imaging system which provides good three-dimensional information. Conventional microscopy does not provide an adequate appreciation of separate events happening at the apical or basolateral surfaces. Moreover, we our work involves imaging dynamic processes in live cells. This requires time lapse capability (4 dimensional imaging) and the minimization of phototoxicity. We have found that multiphoton confocal microscopy works best for our needs.
This Aims of this Core are to: 1. Provide a core facility for a multiphoton confocal microscope, suitably equipped for time lapse imagining of epithelial cells and interaction of epithelial cells with inflammatory cells. 2. Provide technical support and instruction for proper maintenance and operation of this microscope and for analysis of the data. The Core contains a new Zeiss 510 Confocal Microscope built on an inverted Zeiss microscope, equipped with a Coherent pulsed laser system for multiphoton microscopy. The system is completely equipped with all of the accessories needed, including all needed objectives, chamber for control of temperature and gas, computers, and software licenses.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
1P01AI053194-01
Application #
6588024
Study Section
Special Emphasis Panel (ZAI1)
Project Start
2002-12-01
Project End
2007-11-30
Budget Start
Budget End
Support Year
1
Fiscal Year
2002
Total Cost
Indirect Cost
Name
University of California San Francisco
Department
Type
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143
Datta, Anirban; Sandilands, Emma; Mostov, Keith E et al. (2017) Fibroblast-derived HGF drives acinar lung cancer cell polarization through integrin-dependent RhoA-ROCK1 inhibition. Cell Signal 40:91-98
Singer, Mark S; Phillips, Joanna J; Lemjabbar-Alaoui, Hassan et al. (2015) SULF2, a heparan sulfate endosulfatase, is present in the blood of healthy individuals and increases in cirrhosis. Clin Chim Acta 440:72-8
Plaks, Vicki; Boldajipour, Bijan; Linnemann, Jelena R et al. (2015) Adaptive Immune Regulation of Mammary Postnatal Organogenesis. Dev Cell 34:493-504
Casbon, Amy-Jo; Reynaud, Damien; Park, Chanhyuk et al. (2015) Invasive breast cancer reprograms early myeloid differentiation in the bone marrow to generate immunosuppressive neutrophils. Proc Natl Acad Sci U S A 112:E566-75
Bucior, Iwona; Tran, Cindy; Engel, Joanne (2014) Assessing Pseudomonas virulence using host cells. Methods Mol Biol 1149:741-55
Bonnans, Caroline; Lohela, Marja; Werb, Zena (2014) Real-time imaging of myeloid cells dynamics in ApcMin/+ intestinal tumors by spinning disk confocal microscopy. J Vis Exp :51916
Tran, Cindy S; Eran, Yoni; Ruch, Travis R et al. (2014) Host cell polarity proteins participate in innate immunity to Pseudomonas aeruginosa infection. Cell Host Microbe 15:636-43
Kwon, Sang-Ho; Liu, Kathleen D; Mostov, Keith E (2014) Intercellular transfer of GPRC5B via exosomes drives HGF-mediated outward growth. Curr Biol 24:199-204
Kim, J H; Chan, C; Elwell, C et al. (2013) Endosulfatases SULF1 and SULF2 limit Chlamydia muridarum infection. Cell Microbiol 15:1560-71
Maltseva, Inna; Chan, Matilda; Kalus, Ina et al. (2013) The SULFs, extracellular sulfatases for heparan sulfate, promote the migration of corneal epithelial cells during wound repair. PLoS One 8:e69642

Showing the most recent 10 out of 80 publications