The emphasis of the expression Core is on providing a uniform set of procedures for protein expression, protein folding and preliminary structural studies, and to provide expertise for measuring protein-protein interactions using a range of equipment available at the Burnham Institute. Included in the scope of this Core are: 1. State-of-the-art protein expression and purification of virulence factors and their host cell targets using high-throughput technologies 2. Large scale purification of isotopically labeled proteins for NMR 3. Preliminary screens for crystallization, diffraction quality and NMR studies. 4. Analysis of protein folding and inhibitor/protein interactions, using SPR, DSC, CD, ITC, stopped-flow fluorimetry. The Expression Core will be utilized for the production of quantities of pure proteins suitable for 3-D structural analysis. In addition, Projects will require substantial use of the Core to generate protein targets for high throughput combinatorial library screening.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Research Program Projects (P01)
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Special Emphasis Panel (ZAI1)
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Sanford-Burnham Medical Research Institute
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