The emphasis of the expression Core is on providing a uniform set of procedures for protein expression, protein folding and preliminary structural studies, and to provide expertise for measuring protein-protein interactions using a range of equipment available at the Burnham Institute. Included in the scope of this Core are: 1. State-of-the-art protein expression and purification of virulence factors and their host cell targets using high-throughput technologies 2. Large scale purification of isotopically labeled proteins for NMR 3. Preliminary screens for crystallization, diffraction quality and NMR studies. 4. Analysis of protein folding and inhibitor/protein interactions, using SPR, DSC, CD, ITC, stopped-flow fluorimetry. The Expression Core will be utilized for the production of quantities of pure proteins suitable for 3-D structural analysis. In addition, Projects will require substantial use of the Core to generate protein targets for high throughput combinatorial library screening.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI055789-02
Application #
7087830
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
Budget Start
2005-07-01
Budget End
2006-06-30
Support Year
2
Fiscal Year
2005
Total Cost
$227,449
Indirect Cost
Name
Sanford-Burnham Medical Research Institute
Department
Type
DUNS #
020520466
City
La Jolla
State
CA
Country
United States
Zip Code
92037
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