The analyses of animal models is complex and labor intensive. This Murine Animal Biosafety Level 3 Core (Core A) is designed to provide labor to all of the projects during experimental procedures that require an increased labor force. A smoothly operating ABSL 3 is critical to this program project and this core will be responsible for maintaining the ABSL and its equipment. The ABSL III facility in this program project is actually a combination lab capable of performing animal and cell culture experiments in it. While each of the projects has its own personnel to concentrate on the goals of that particular project, the maintenance, upkeep and labor intensity of the projects requires additional personnel that can assure that this core lab is maintained at a high level and assist with the labor intensive animal models and organ collection. There are many methods particularly related to the animal experiments that overlap among the different projects. Therefore having two experienced personnel that can enter into the animal experiments at critical times will be both cost effective and efficient as well as maintain quality control among the animal experiments. The role of Core A is:
Aim 1. Maintain stocks and inoculation procedures to maintain reproducible models.
Aim 2. Maintain compliance within the ABSL 3 lab for excellent biosafety and select agent status.
Aim 3. Assist technicians assigned to projects in the labor intensive portion of animal experiments in Projects 1, 2 and 3.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI056295-05
Application #
7883573
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
Budget Start
2009-07-01
Budget End
2010-06-30
Support Year
5
Fiscal Year
2009
Total Cost
$237,619
Indirect Cost
Name
University of New Mexico
Department
Type
DUNS #
868853094
City
Albuquerque
State
NM
Country
United States
Zip Code
87131
Hutt, Julie A; Lovchik, Julie A; Drysdale, Melissa et al. (2014) Lethal factor, but not edema factor, is required to cause fatal anthrax in cynomolgus macaques after pulmonary spore challenge. Am J Pathol 184:3205-16
Lovchik, Julie A; Drysdale, Melissa; Koehler, Theresa M et al. (2012) Expression of either lethal toxin or edema toxin by Bacillus anthracis is sufficient for virulence in a rabbit model of inhalational anthrax. Infect Immun 80:2414-25
Mara-Koosham, Gopi; Hutt, Julie A; Lyons, C Rick et al. (2011) Antibodies contribute to effective vaccination against respiratory infection by type A Francisella tularensis strains. Infect Immun 79:1770-8
Hansen, Spencer J; Rushton, John; Dekonenko, Alexander et al. (2011) Cowpox virus inhibits human dendritic cell immune function by nonlethal, nonproductive infection. Virology 412:411-25
Borovkov, Alex Y; Loskutov, Andrey V; Robida, Mark D et al. (2010) High-quality gene assembly directly from unpurified mixtures of microarray-synthesized oligonucleotides. Nucleic Acids Res 38:e180
Chand, Hitendra S; Schuyler, Mark; Joste, Nancy et al. (2010) Anti-IgE therapy results in decreased myeloid dendritic cells in asthmatic airways. J Allergy Clin Immunol 125:1157-1158.e5
Lipscomb, Mary F; Hutt, Julie; Lovchik, Julie et al. (2010) The pathogenesis of acute pulmonary viral and bacterial infections: investigations in animal models. Annu Rev Pathol 5:223-52
Wu, Terry H; Zsemlye, Jason L; Statom, Gloria L et al. (2009) Vaccination of Fischer 344 rats against pulmonary infections by Francisella tularensis type A strains. Vaccine 27:4684-93
Collazo, Carmen M; Meierovics, Anda I; De Pascalis, Roberto et al. (2009) T cells from lungs and livers of Francisella tularensis-immune mice control the growth of intracellular bacteria. Infect Immun 77:2010-21
Hahn, Andrew C; Lyons, C Rick; Lipscomb, Mary F (2008) Effect of Bacillus anthracis virulence factors on human dendritic cell activation. Hum Immunol 69:552-61

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