Abstract

Virologic studies for this program will be performed at two different sites. 1.) Quantitation of HIV-RNA by the ultra sensitive Roche RT-PCR Amplicor assay and genotyping for drug resistance will be done in our high through-put Bellevue Hospital laboratory. Measurements of HIV by real time PCR for rapid diagnosis of individuals at the earliest stages of acute infection, and heteroduplex gel tracking assays to monitor viral evolution will be performed in the Borkowsky CFAR laboratory at Bellevue Hospital. 2.) Total genomic sequencing of HIV isolates from multiple time points in up to 15 patients per year, and the """"""""detuned"""""""" ELISA assays will be performed. All projects in this program will correlate their immunological data with the viral load measurements and HIV-genomic sequences provided by this core. Studies of the evolution of CD4 responses will be compared with the evolution of the subjects' HIV as documented by heteroduplex analyses, by sequencing, and by mapping changes in epitopes of the viruses with the peptides recognized by the CD4 cells.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI057127-05
Application #
7575655
Study Section
Acquired Immunodeficiency Syndrome Research Review Committee (AIDS)
Project Start
2008-03-01
Project End
2009-02-28
Budget Start
2008-03-01
Budget End
2009-02-28
Support Year
5
Fiscal Year
2008
Total Cost
$190,876
Indirect Cost

  Institution

Name
New York University
Department
Type
DUNS #
121911077
City
New York
State
NY
Country
United States
Zip Code
10016

  Publications

Gornalusse, German G; Mummidi, Srinivas; Gaitan, Alvaro A et al. (2015) Epigenetic mechanisms, T-cell activation, and CCR5 genetics interact to regulate T-cell expression of CCR5, the major HIV-1 coreceptor. Proc Natl Acad Sci U S A 112:E4762-71
Miller, Elizabeth A; Spadaccia, Meredith R; Norton, Thomas et al. (2015) Attenuated Listeria monocytogenes vectors overcome suppressive plasma factors during HIV infection to stimulate myeloid dendritic cells to promote adaptive immunity and reactivation of latent virus. AIDS Res Hum Retroviruses 31:127-36
Miller, Elizabeth; Spadaccia, Meredith; Sabado, Rachel et al. (2015) Autologous aldrithiol-2-inactivated HIV-1 combined with polyinosinic-polycytidylic acid-poly-L-lysine carboxymethylcellulose as a vaccine platform for therapeutic dendritic cell immunotherapy. Vaccine 33:388-95
Miller, Elizabeth; Bhardwaj, Nina (2013) Dendritic cell dysregulation during HIV-1 infection. Immunol Rev 254:170-89
Koblin, Beryl A; Mayer, Kenneth H; Noonan, Elizabeth et al. (2012) Sexual risk behaviors, circumcision status, and preexisting immunity to adenovirus type 5 among men who have sex with men participating in a randomized HIV-1 vaccine efficacy trial: step study. J Acquir Immune Defic Syndr 60:405-13
Miller, Elizabeth A; Spadaccia, Meredith R; O?Brien, Meagan P et al. (2012) Plasma factors during chronic HIV-1 infection impair IL-12 secretion by myeloid dendritic cells via a virus-independent pathway. J Acquir Immune Defic Syndr 61:535-44
Stein, Dylan; Silvera, Richard; Hagerty, Robert et al. (2012) Viewing pornography depicting unprotected anal intercourse: are there implications for HIV prevention among men who have sex with men? Arch Sex Behav 41:411-9
O'Brien, Meagan; Manches, Olivier; Sabado, Rachel Lubong et al. (2011) Spatiotemporal trafficking of HIV in human plasmacytoid dendritic cells defines a persistently IFN-?-producing and partially matured phenotype. J Clin Invest 121:1088-101
Sabado, Rachel Lubong; O'Brien, Meagan; Subedi, Abhignya et al. (2010) Evidence of dysregulation of dendritic cells in primary HIV infection. Blood 116:3839-52
El Hed, Aimee; Khaitan, Alka; Kozhaya, Lina et al. (2010) Susceptibility of human Th17 cells to human immunodeficiency virus and their perturbation during infection. J Infect Dis 201:843-54

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