Abstract

The Core (Core B) includes Beckman FacScan flow cytometer that will be used extensively bymembers of the program project. The facility will be directed by Dr. Yufang Shi. The facility will be directed by Dr. Yufang Shi. All members of the Program Project will use the instrument without charge for facility use. Other investigators pay an annual user fee of $400 to helpcover the cost of the Service Contract. The director will train members of the Program Project and any of their staff at periodic training sessions. The facility will cover 50 % of the cost of Dr. Jina-Nian Zhou who will care for the Flow Cytometer and provide the data (graphs, analysis) to the investigators. Appropriate supplies will be provide by the program to cover the costs of its use. Investigators who wish to use the facility will be trained by the director of the facility and/or his staff. All members of the program project will be using this facility extensively to measure levels of mRNA in cells.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI057596-02
Application #
7098826
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
Budget Start
2005-07-01
Budget End
2006-06-30
Support Year
2
Fiscal Year
2005
Total Cost
$28,045
Indirect Cost

  Institution

Name
University of Medicine & Dentistry of NJ
Department
Type
DUNS #
617022384
City
Piscataway
State
NJ
Country
United States
Zip Code
08854

  Publications

Krause, Christopher D; Izotova, Lara S; Pestka, Sidney (2013) Analytical use of multi-protein Fluorescence Resonance Energy Transfer to demonstrate membrane-facilitated interactions within cytokine receptor complexes. Cytokine 64:298-309
Zhang, J; Roberts, A I; Liu, C et al. (2013) A novel subset of helper T cells promotes immune responses by secreting GM-CSF. Cell Death Differ 20:1731-41
Krause, Christopher D; Digioia, Gina; Izotova, Lara S et al. (2013) Improving the spectral analysis of Fluorescence Resonance Energy Transfer in live cells: application to interferon receptors and Janus kinases. Cytokine 64:272-85
Krause, Christopher D; Digioia, Gina; Izotova, Lara S et al. (2013) Ligand-independent interaction of the type I interferon receptor complex is necessary to observe its biological activity. Cytokine 64:286-97
Wu, Xiangyue; Chesoni, Sandra; Rondeau, Gaelle et al. (2013) Combinatorial mRNA binding by AUF1 and Argonaute 2 controls decay of selected target mRNAs. Nucleic Acids Res 41:2644-58
Krause, Christopher D; Izotova, Lara S; Ren, Gwangwen et al. (2011) Efficient co-expression of bicistronic proteins in mesenchymal stem cells by development and optimization of a multifunctional plasmid. Stem Cell Res Ther 2:15
Matus-Nicodemos, Rodrigo; Vavassori, Stefano; Castro-Faix, Moraima et al. (2011) Polypyrimidine tract-binding protein is critical for the turnover and subcellular distribution of CD40 ligand mRNA in CD4+ T cells. J Immunol 186:2164-71
Yamaza, T; Ren, G; Akiyama, K et al. (2011) Mouse mandible contains distinctive mesenchymal stem cells. J Dent Res 90:317-24
Sarkar, Srijata; Han, Junfeng; Sinsimer, Kristina S et al. (2011) RNA-binding protein AUF1 regulates lipopolysaccharide-induced IL10 expression by activating IkappaB kinase complex in monocytes. Mol Cell Biol 31:602-15
Knapinska, Anna M; Gratacós, Frances M; Krause, Christopher D et al. (2011) Chaperone Hsp27 modulates AUF1 proteolysis and AU-rich element-mediated mRNA degradation. Mol Cell Biol 31:1419-31

Showing the most recent 10 out of 36 publications