The Protein Expression, Purification and Interaction Core (Core C) will provide instrumentation, expertise and service related to the cloning, over expression and purification of viral proteins and virus-like-particles (VLPs) as needed by each of the three project components. In addition, the Core will utilize the purified proteins and VLPs to measure interactions with other relevant ligands using surface plasmon resonance. This core will provide the following specific functions: (1) cloning and sequencing of wild-type and mutant genes encoding viral proteins into bacterial and insect cell protein expression vectors; (2) optimization of protein expression levels in the bacterial and insect systems; (3) purification of viral proteins; (4) expression, characterization and purification of VLPs; (5) quantitation of protein interactions with antibodies, RNA and proteins, polysaccharides and other viral proteins; and (6) synthesis of peptide arrays for fine mapping of monoclonal antibody epitopes.
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| Costantini, Veronica; Morantz, Esther K; Browne, Hannah et al. (2018) Human Norovirus Replication in Human Intestinal Enteroids as Model to Evaluate Virus Inactivation. Emerg Infect Dis 24:1453-1464 |
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| Ramani, Sasirekha; Neill, Frederick H; Ferreira, Jennifer et al. (2017) B-Cell Responses to Intramuscular Administration of a Bivalent Virus-Like Particle Human Norovirus Vaccine. Clin Vaccine Immunol 24: |
| Hurwitz, Amy M; Huang, Wanzhi; Estes, Mary K et al. (2017) Deep sequencing of phage-displayed peptide libraries reveals sequence motif that detects norovirus. Protein Eng Des Sel 30:129-139 |
| Sharma, Sumit; Carlsson, Beatrice; Czakó, Rita et al. (2017) Human Sera Collected between 1979 and 2010 Possess Blocking-Antibody Titers to Pandemic GII.4 Noroviruses Isolated over Three Decades. J Virol 91: |
| Shanker, Sreejesh; Hu, Liya; Ramani, Sasirekha et al. (2017) Structural features of glycan recognition among viral pathogens. Curr Opin Struct Biol 44:211-218 |
| Zou, Winnie Y; Blutt, Sarah E; Crawford, Sue E et al. (2017) Human Intestinal Enteroids: New Models to Study Gastrointestinal Virus Infections. Methods Mol Biol : |
| Yu, Huimin; Hasan, Nesrin M; In, Julie G et al. (2017) The Contributions of Human Mini-Intestines to the Study of Intestinal Physiology and Pathophysiology. Annu Rev Physiol 79:291-312 |
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