Abstract

Francisella tularensis is a highly infectious pathogen that can be acquired by the aerosol route. As such, it has been classified as a Category A select agent by the CDC. No licensed vaccine is available for protection against infection by this organism. Genomic approaches, relying heavily on microarray development and analysis, will be key to unlocking the mysteries concerning the virulence of the organism, as well as the response of the host, during infection. This Core will be focused on support for all of the Projects in this PO1 application with the following aims:
Aim 1 is construction and utilization of a F. tularensis Schu4 oligonucleotide microarray; and, Aim 2 is support services to the program project for DNA sequencing, oligonucleotide production, and other nucleic acid detection/analytical functions (e.g., Real Time PCR and Typhoon Imaging system). With the support of this Core, the individual projects will lead to novel discoveries in the fields of Francisella pathogenesis and immunity.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
1P01AI057986-01A2
Application #
6912417
Study Section
Special Emphasis Panel (ZAI1-EC-M (J1))
Project Start
2005-07-08
Project End
2010-06-30
Budget Start
2005-07-08
Budget End
2006-06-30
Support Year
1
Fiscal Year
2005
Total Cost
$73,080
Indirect Cost

  Institution

Name
University of Texas Health Science Center San Antonio
Department
Type
DUNS #
800189185
City
San Antonio
State
TX
Country
United States
Zip Code
78249

  Publications

Nguyen, Jesse Q; Gilley, Ryan P; Zogaj, Xhavit et al. (2014) Lipidation of the FPI protein IglE contributes to Francisella tularensis ssp. novicida intramacrophage replication and virulence. Pathog Dis 72:10-8
Chu, Ping; Cunningham, Aimee L; Yu, Jieh-Juen et al. (2014) Live attenuated Francisella novicida vaccine protects against Francisella tularensis pulmonary challenge in rats and non-human primates. PLoS Pathog 10:e1004439
Tsai, Su-Yu; Segovia, Jesus A; Chang, Te-Hung et al. (2014) DAMP molecule S100A9 acts as a molecular pattern to enhance inflammation during influenza A virus infection: role of DDX21-TRIF-TLR4-MyD88 pathway. PLoS Pathog 10:e1003848
Zogaj, Xhavit; Wyatt, Geoff C; Klose, Karl E (2012) Cyclic di-GMP stimulates biofilm formation and inhibits virulence of Francisella novicida. Infect Immun 80:4239-47
Rodriguez, Annette R; Yu, Jieh-Juen; Guentzel, M Neal et al. (2012) Mast cell TLR2 signaling is crucial for effective killing of Francisella tularensis. J Immunol 188:5604-11
Sanapala, Shilpa; Yu, Jieh-Juen; Murthy, Ashlesh K et al. (2012) Perforin- and granzyme-mediated cytotoxic effector functions are essential for protection against Francisella tularensis following vaccination by the defined F. tularensis subsp. novicida ýýfopC vaccine strain. Infect Immun 80:2177-85
Boyd, Angela R; Shivshankar, Pooja; Jiang, Shoulei et al. (2012) Age-related defects in TLR2 signaling diminish the cytokine response by alveolar macrophages during murine pneumococcal pneumonia. Exp Gerontol 47:507-18
Signarovitz, Aimee L; Ray, Heather J; Yu, Jieh-Juen et al. (2012) Mucosal immunization with live attenuated Francisella novicida U112ýýiglB protects against pulmonary F. tularensis SCHU S4 in the Fischer 344 rat model. PLoS One 7:e47639
Arulanandam, Bernard P; Chetty, Senthilnath Lakshmana; Yu, Jieh-Juen et al. (2012) Francisella DnaK inhibits tissue-nonspecific alkaline phosphatase. J Biol Chem 287:37185-94
Hunter, Colleen; Rodriguez, Annette; Yu, Jieh-Juen et al. (2012) Comparison of bone marrow-derived and mucosal mast cells in controlling intramacrophage Francisella tularensis replication. Exp Biol Med (Maywood) 237:617-21

Showing the most recent 10 out of 49 publications