This Program Project conducts a comprehensive analysis of the mechanism(s) of HIV latency and viral reservoirs. In vitro investigations in tissue culture systems (Projects 1, 2, and 3) will be closely coordinated with in vivo studies in this core (""""""""Non-human Primates for HIV/SIV Reservoirs and Latency""""""""). Accordingly, this core will provide rhesus macaques and appropriate infrastructure support required for the animal model studies in this Program Project. This Core uses macaques housed at the California National Primate Research Center (CNPRC) at UC Davis. This Center is accredited by the American Association for Accreditation of Laboratory Animal Care (AAALAC) and includes full-time, licensed veterinarians. Appropriate facilities and expertise are available for the care and handling of macaques inoculated with SIV/SHIV at biosafety levels 2 and 3 (BSL-2, -3). In addition, the CNPRC infrastructure and staff provide for clinical analysis of infected animals; this includes physical diagnosis, microbiological analysis, and hematological assessments. Veterinary pathologists perform comprehensive necropsy and pathology analysis and to collect tissues and organs for all of the projects in this Program. Several studies on antiretroviral drugs, in the SIV/macaque and RT-SHIV/macaque systems, have been conducted at the CNPRC; thus, this Core builds on demonstrated strengths that are directly relevant to the goals of the Program Project. For the studies in Core B, we hypothesize that the macaque model of SIV/SHIV infection can be used to define viral reservoirs, to analyze latency, and to study activation of virus in vivo.
Three Specific Aims address this hypothesis:
Aim 1 : to define conditions in the SIV/macaque and RT-SHIV/macaque systems for analysis of viral latency/reservoirs as observed in humans infected with HIV;
Aim 2 : to analyze virus localization in infected macaques undergoing antiretroviral therapy;
Aim 3 : to determine the role of viral and host-cell factors in the regulation of viral latency, persistence, and reactivation.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Research Program Projects (P01)
Project #
Application #
Study Section
Acquired Immunodeficiency Syndrome Research Review Committee (AIDS)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
J. David Gladstone Institutes
San Francisco
United States
Zip Code
Pak, Vladimir; Eifler, Tristan T; Jäger, Stefanie et al. (2015) CDK11 in TREX/THOC Regulates HIV mRNA 3' End Processing. Cell Host Microbe 18:560-70
Wadford, Debra A; Kauffman, Robert C; Deere, Jesse D et al. (2014) Variation of human immunodeficiency virus type-1 reverse transcriptase within the simian immunodeficiency virus genome of RT-SHIV. PLoS One 9:e86997
Deere, Jesse D; Kauffman, Robert C; Cannavo, Elda et al. (2014) Analysis of multiply spliced transcripts in lymphoid tissue reservoirs of rhesus macaques infected with RT-SHIV during HAART. PLoS One 9:e87914
Chan, Jonathan K; Bhattacharyya, Darshana; Lassen, Kara G et al. (2013) Calcium/calcineurin synergizes with prostratin to promote NF-?B dependent activation of latent HIV. PLoS One 8:e77749
Hakre, Shweta; Chavez, Leonard; Shirakawa, Kotaro et al. (2011) Epigenetic regulation of HIV latency. Curr Opin HIV AIDS 6:19-24
Chan, Jonathan K L; Greene, Warner C (2011) NF-ýýB/Rel: agonist and antagonist roles in HIV-1 latency. Curr Opin HIV AIDS 6:12-8
Lenasi, Tina; Peterlin, B Matija; Barboric, Matjaz (2011) Cap-binding protein complex links pre-mRNA capping to transcription elongation and alternative splicing through positive transcription elongation factor b (P-TEFb). J Biol Chem 286:22758-68
Deere, Jesse D; Schinazi, Raymond F; North, Thomas W (2011) Simian immunodeficiency virus macaque models of HIV latency. Curr Opin HIV AIDS 6:57-61
North, Thomas W; Higgins, Joanne; Deere, Jesse D et al. (2010) Viral sanctuaries during highly active antiretroviral therapy in a nonhuman primate model for AIDS. J Virol 84:2913-22
Deere, Jesse D; Higgins, Joanne; Cannavo, Elda et al. (2010) Viral decay kinetics in the highly active antiretroviral therapy-treated rhesus macaque model of AIDS. PLoS One 5:e11640

Showing the most recent 10 out of 23 publications