Abstract

Candida albicans is the most frequently isolated fungal pathogen of humans. It is a common pathogen that causes bloodstream infection in hospitals, with estimated mortality at 40%. Increased use of prophylactic antifungal drugs in high-risk patients accelerates the development of drug resistance. Alternative antifungal therapies are urgently needed. One promising approach to avoid anti-fungal resistance is to use immune-based therapies. Protective antibodies against C. albicans heat shock protein 90 (Hsp90) or beta-mannan have been shown to be active against hematogenously disseminated candidiasis in experimental mice. In particular, a human genetically recombinant antibody against the Hsp90 is currently in a multinational trial in patients with disseminated candidiasis. To identify potential vaccines and protective antibodies, we propose to develop a C. albicans protein microarray to profile humoral responses in a large number of patients with invasive candidiasis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI061537-04
Application #
7435285
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
Budget Start
2007-06-01
Budget End
2008-05-31
Support Year
4
Fiscal Year
2007
Total Cost
$51,390
Indirect Cost

  Institution

Name
University of Florida
Department
Type
DUNS #
969663814
City
Gainesville
State
FL
Country
United States
Zip Code
32611

  Publications

Cartmell, Jonathan; Paszkiewicz, Eugenia; Dziadek, Sebastian et al. (2015) Synthesis of antifungal vaccines by conjugation of ?-1,2 trimannosides with T-cell peptides and covalent anchoring of neoglycopeptide to tetanus toxoid. Carbohydr Res 403:123-34
Du, Chen; Wingard, John R; Cheng, Shaoji et al. (2012) Serum IgG responses against Aspergillus proteins before hematopoietic stem cell transplantation or chemotherapy identify patients who develop invasive aspergillosis. Biol Blood Marrow Transplant 18:1927-34
Nguyen, M Hong; Leather, Helen; Clancy, Cornelius J et al. (2011) Galactomannan testing in bronchoalveolar lavage fluid facilitates the diagnosis of invasive pulmonary aspergillosis in patients with hematologic malignancies and stem cell transplant recipients. Biol Blood Marrow Transplant 17:1043-50
Cutler, Jim E; Corti, Miriam; Lambert, Patrick et al. (2011) Horizontal transmission of Candida albicans and evidence of a vaccine response in mice colonized with the fungus. PLoS One 6:e22030
Vadnerkar, Aniket; Toyoda, Yoshiya; Crespo, Maria et al. (2011) Age-specific complications among lung transplant recipients 60 years and older. J Heart Lung Transplant 30:273-81
Vadnerkar, Aniket; Clancy, Cornelius J; Celik, Umit et al. (2010) Impact of mold infections in explanted lungs on outcomes of lung transplantation. Transplantation 89:253-60
Mitsani, Dimitra; Nguyen, M Hong; Kwak, Eun J et al. (2010) Cytomegalovirus disease among donor-positive/recipient-negative lung transplant recipients in the era of valganciclovir prophylaxis. J Heart Lung Transplant 29:1014-20
Mochon, A Brian; Jin, Ye; Ye, Jin et al. (2010) Serological profiling of a Candida albicans protein microarray reveals permanent host-pathogen interplay and stage-specific responses during candidemia. PLoS Pathog 6:e1000827
Vadnerkar, Aniket; Nguyen, M Hong; Mitsani, Dimitra et al. (2010) Voriconazole exposure and geographic location are independent risk factors for squamous cell carcinoma of the skin among lung transplant recipients. J Heart Lung Transplant 29:1240-4
Hao, Binghua; Clancy, Cornelius J; Cheng, Shaoji et al. (2009) Candida albicans RFX2 encodes a DNA binding protein involved in DNA damage responses, morphogenesis, and virulence. Eukaryot Cell 8:627-39

Showing the most recent 10 out of 26 publications