Abstract

Two-photon imaging can provide dynamic information about the behavior of pathogens and immune cells in tissues during infection. Such information can provide unique insight into the course of events during infection and the impact of therapeutic treatments. However, this technique is outside the reach of many investigators due to the cost of purchasing and maintaining equipment, and the challenges in preparing samples and analyzing data. In this proposal we plan to make this powerful tool available to the investigators of the PO1 by: (1) working with individual labs on pilot studies to determine the optimal experimental set-ups to meet individual imaging goal, (2) expanding existing imaging equipment to provide more access, and (3) assisting individual labs to make use of quantitation software and perform mathematical analysis of visual data.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI065831-05
Application #
8113269
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
Budget Start
2010-07-01
Budget End
2011-06-30
Support Year
5
Fiscal Year
2010
Total Cost
$241,473
Indirect Cost

  Institution

Name
University of California Berkeley
Department
Type
DUNS #
124726725
City
Berkeley
State
CA
Country
United States
Zip Code
94704

  Publications

Chu, H Hamlet; Chan, Shiao-Wei; Gosling, John Paul et al. (2016) Continuous Effector CD8(+) T Cell Production in a Controlled Persistent Infection Is Sustained by a Proliferative Intermediate Population. Immunity 45:159-71
Han, Seong-Ji; Melichar, Heather J; Coombes, Janine L et al. (2014) Internalization and TLR-dependent type I interferon production by monocytes in response to Toxoplasma gondii. Immunol Cell Biol 92:872-81
Grover, Harshita Satija; Chu, H Hamlet; Kelly, Felice D et al. (2014) Impact of regulated secretion on antiparasitic CD8 T cell responses. Cell Rep 7:1716-1728
Young, Jennifer A; He, Tina H; Reizis, Boris et al. (2013) Commensal microbiota are required for systemic inflammation triggered by necrotic dendritic cells. Cell Rep 3:1932-44
Coombes, Janine L; Charsar, Brittany A; Han, Seong-Ji et al. (2013) Motile invaded neutrophils in the small intestine of Toxoplasma gondii-infected mice reveal a potential mechanism for parasite spread. Proc Natl Acad Sci U S A 110:E1913-22
Grover, Harshita Satija; Blanchard, Nicolas; Gonzalez, Federico et al. (2012) The Toxoplasma gondii peptide AS15 elicits CD4 T cells that can control parasite burden. Infect Immun 80:3279-88
Coombes, Janine L; Han, Seong-Ji; van Rooijen, Nico et al. (2012) Infection-induced regulation of natural killer cells by macrophages and collagen at the lymph node subcapsular sinus. Cell Rep 2:124-35
Dzhagalov, Ivan L; Melichar, Heather J; Ross, Jenny O et al. (2012) Two-photon imaging of the immune system. Curr Protoc Cytom Chapter 12:Unit12.26
Young, Jennifer A; Sermwittayawong, Decha; Kim, Hee-Jung et al. (2011) Fas-associated death domain (FADD) and the E3 ubiquitin-protein ligase TRIM21 interact to negatively regulate virus-induced interferon production. J Biol Chem 286:6521-31
Tam, Connie; LeDue, Jeffrey; Mun, James J et al. (2011) 3D quantitative imaging of unprocessed live tissue reveals epithelial defense against bacterial adhesion and subsequent traversal requires MyD88. PLoS One 6:e24008

Showing the most recent 10 out of 17 publications