Abstract

The overall goal of the Immunology & Virology Core is to provide a centralized resource for detailed analysis of cellular and humoral immune responses for all investigators in this HIVRAD project. The Immunology & Virology Core will assist in the planning of assays, carry out assays of cellular and humoral immune responses for animals from multiple different projects, thus allowing accurate comparisons of immune responses among different experimental groups, serve as central repository for common reagents and experimental protocols, and assist in the interpretation and presentation of immunologic data. Specific assays that will be provided by the Immunology & Virology Core include standard and polychromatic flow cytometric analysis; analysis of cell-mediated immune responses using MHC tetramers, ELISPOT and intracellular cytokine staining; analysis of humoral immune responses using ELISA assays, Western blot and SIV neutralization assays; MHC class I typing by PCR; and preparation of virus stocks for challenge and neutralization assays.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
1P01AI071306-01A1
Application #
7294526
Study Section
Special Emphasis Panel (ZAI1-MPM-A (J1))
Project Start
2007-01-01
Project End
2012-03-31
Budget Start
2007-04-15
Budget End
2008-03-31
Support Year
1
Fiscal Year
2007
Total Cost
$503,291
Indirect Cost

  Institution

Name
Harvard University
Department
Type
DUNS #
047006379
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Shang, L; Smith, A J; Reilly, C S et al. (2018) Vaccine-modified NF-kB and GR signaling in cervicovaginal epithelium correlates with protection. Mucosal Immunol 11:512-522
Shang, L; Duan, L; Perkey, K E et al. (2017) Epithelium-innate immune cell axis in mucosal responses to SIV. Mucosal Immunol 10:508-519
Zeng, Ming; Smith, Anthony J; Shang, Liang et al. (2016) Mucosal Humoral Immune Response to SIVmac239?nef Vaccination and Vaginal Challenge. J Immunol 196:2809-18
Adnan, Sama; Colantonio, Arnaud D; Yu, Yi et al. (2015) CD8 T cell response maturation defined by anentropic specificity and repertoire depth correlates with SIV?nef-induced protection. PLoS Pathog 11:e1004633
George, Michael D; Hu, William; Billingsley, James M et al. (2014) Transcriptional profiling of peripheral CD8+T cell responses to SIV?nef and SIVmac251 challenge reveals a link between protective immunity and induction of systemic immunoregulatory mechanisms. Virology 468-470:581-91
Li, Qingsheng; Zeng, Ming; Duan, Lijie et al. (2014) Live simian immunodeficiency virus vaccine correlate of protection: local antibody production and concentration on the path of virus entry. J Immunol 193:3113-25
Smith, Anthony J; Wietgrefe, Stephen W; Shang, Liang et al. (2014) Live simian immunodeficiency virus vaccine correlate of protection: immune complex-inhibitory Fc receptor interactions that reduce target cell availability. J Immunol 193:3126-33
Shang, Liang; Smith, Anthony J; Duan, Lijie et al. (2014) NK cell responses to simian immunodeficiency virus vaginal exposure in naive and vaccinated rhesus macaques. J Immunol 193:277-84
Manrique, Julieta; Piatak, Michael; Lauer, William et al. (2013) Influence of mismatch of Env sequences on vaccine protection by live attenuated simian immunodeficiency virus. J Virol 87:7246-54
Rahmberg, Andrew R; Neidermyer Jr, William J; Breed, Matthew W et al. (2013) Tetherin upregulation in simian immunodeficiency virus-infected macaques. J Virol 87:13917-21

Showing the most recent 10 out of 31 publications