The purpose of Core C is to provide support to the Projects and Core B for all high biocontainment work. The core brings unique expertise in molecular biology and cell biology of Ebola viruses. It also has high throughput assay capacity for measuring and quantitating virus infection efficiency that forms part of prioritization of host factors for follow up work in the other projects. The core will acquire, cultivate and characterize Ebola virus isolates that include those from the 2014 West Africa outbreak as well as make recombinant viruses containing amino acid changes that are produced from a reverse genetics system. The core will supply nucleic acids and cell lysates from viruses and infected cells. After host factors are identified and virus protein critical residues are known, from the Projects, each will be validated using cell lines infected with wild type and recombinant viruses. Animal work to evaluate pathogenesis will be performed using mouse models of disease. Each of these services supports key experiments performed in each Project ensuring that Core C will interact heavily with each part and be a key component of this Program Project.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
1P01AI120943-01A1
Application #
9149558
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
Budget Start
2016-07-07
Budget End
2017-06-30
Support Year
1
Fiscal Year
2016
Total Cost
Indirect Cost
Name
Washington University
Department
Type
DUNS #
068552207
City
Saint Louis
State
MO
Country
United States
Zip Code
63130
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Hung, Putzer J; Johnson, Britney; Chen, Bo-Ruei et al. (2018) MRI Is a DNA Damage Response Adaptor during Classical Non-homologous End Joining. Mol Cell 71:332-342.e8
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Dashti, Hesam; Wedell, Jonathan R; Westler, William M et al. (2018) Applications of Parametrized NMR Spin Systems of Small Molecules. Anal Chem 90:10646-10649

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