Abstract

This is a renewal grant application for an ongoing program project whose overall objective is the study of the chemistry and metabolism of connective tissues. The program is composed of five projects and two core units. Project 1 will deal with the genetic regulation of elastin biosynthesis and metabolism. Project 2 will explore the mechanisms of post-translational glycosylation of different collagens and the properties and formation of matrix glycoproteins. Project 3 will continue to study the biochemistry and immunology of basement membranes and will extend this work into a study of genetic regulation. Project 4 will investigate the properties of basement membrane proteins involved in the pathogenesis of interstitial nephritis. Project 5 will extend the knowledge gained from the study of connective tissue components into the properties of cells generating these proteins and in comparing properties of cells from different tissues during differentiation. An administrative core and a core analytical facility will supplement the research efforts.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Program Projects (P01)
Project #
5P01AR020553-11
Application #
3092337
Study Section
(SRC)
Project Start
1978-05-01
Project End
1988-04-30
Budget Start
1987-05-01
Budget End
1988-04-30
Support Year
11
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
University City Science Center
Department
Type
DUNS #
City
Philadelphia
State
PA
Country
United States
Zip Code
19104

  Publications

Myers, Jeanne C; Amenta, Peter S; Dion, Arnold S et al. (2007) The molecular structure of human tissue type XV presents a unique conformation among the collagens. Biochem J 404:535-44
Shahan, Tracy; Grant, Derrick; Tootell, Mason et al. (2004) Oncothanin, a peptide from the alpha3 chain of type IV collagen, modifies endothelial cell function and inhibits angiogenesis. Connect Tissue Res 45:151-63
Myers, Jeanne C; Li, Deqin; Amenta, Peter S et al. (2003) Type XIX collagen purified from human umbilical cord is characterized by multiple sharp kinks delineating collagenous subdomains and by intermolecular aggregates via globular, disulfide-linked, and heparin-binding amino termini. J Biol Chem 278:32047-57
Fawzi, A; Robinet, A; Monboisse, J C et al. (2000) A peptide of the alpha 3(IV) chain of type IV collagen modulates stimulated neutrophil function via activation of cAMP-dependent protein kinase and Ser/Thr protein phosphatase. Cell Signal 12:327-35
Shahan, T A; Fawzi, A; Bellon, G et al. (2000) Regulation of tumor cell chemotaxis by type IV collagen is mediated by a Ca(2+)-dependent mechanism requiring CD47 and the integrin alpha(V)beta(3). J Biol Chem 275:4796-802
Pasco, S; Han, J; Gillery, P et al. (2000) A specific sequence of the noncollagenous domain of the alpha3(IV) chain of type IV collagen inhibits expression and activation of matrix metalloproteinases by tumor cells. Cancer Res 60:467-73
Shahan, T A; Ohno, N; Pasco, S et al. (1999) Inhibition of tumor cell proliferation by type IV collagen requires increased levels of cAMP. Connect Tissue Res 40:221-32
Shahan, T A; Ziaie, Z; Pasco, S et al. (1999) Identification of CD47/integrin-associated protein and alpha(v)beta3 as two receptors for the alpha3(IV) chain of type IV collagen on tumor cells. Cancer Res 59:4584-90
Han, J; Jenq, W; Kefalides, N A (1999) Integrin alpha2beta1 recognizes laminin-2 and induces C-erb B2 tyrosine phosphorylation in metastatic human melanoma cells. Connect Tissue Res 40:283-93
Zhang, Y; Niu, Z; Cohen, A J et al. (1999) The internal chondrocyte-specific promoter of the chick type III collagen gene is activated by AP1 and is repressed in fibroblasts by a complex containing an LBP1-related protein. Nucleic Acids Res 27:4090-9

Showing the most recent 10 out of 93 publications