The Dermatology Animal Core (DAC). DAC was established in 1996 to generate transgenic mice of dermatological interest. Resources include a full microinjection suite for both pronuclear and blastocyst injections and the mice necessary to generate transgenic animals using, these protocols. Typically, we use FVB/N or CD- 1 mice for pronuclear injection. C57BL/6J mice are used as donor blastocysts for ES microinjection. Either FVB/N or CD- 1 vasectornized males are used to mate with B6CBAF1 (F1 generation of a cross between B6 and CBA mice) females to generate the pseudopregnant foster mothers. Currently, DAC consists of I faculty member (John F. Klement; 30% effort) as the director and 1 technician. By itself, or with DNA targeting vectors generated in other labs, DAC has successfully generated three knock-out lines. These include the collagen type VII null mice, which serves as a model for recessive dystrophic epidermolysis bullosa, and also periplakin, and ladinin. In collaboration with members of the Department of Dermatology, who generated the recombinant ES cells, DAC generated three other gene ablation mouse lines. Currently, DAC has several projects in progress. DAC has successfully generated several mouse lines by pronuclear injection. These mice are currently being analyzed.

Project Start
2002-04-01
Project End
2007-03-31
Budget Start
Budget End
Support Year
16
Fiscal Year
2002
Total Cost
Indirect Cost
Name
Thomas Jefferson University
Department
Type
DUNS #
061197161
City
Philadelphia
State
PA
Country
United States
Zip Code
19107
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Chung, Hye Jin; Uitto, Jouni (2010) Type VII collagen: the anchoring fibril protein at fault in dystrophic epidermolysis bullosa. Dermatol Clin 28:93-105
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Varki, Roslyn; Sadowski, Sara; Uitto, Jouni et al. (2007) Epidermolysis bullosa. II. Type VII collagen mutations and phenotype-genotype correlations in the dystrophic subtypes. J Med Genet 44:181-92
Nyquist, Gurston G; Mumm, Christina; Grau, Renee et al. (2007) Malignant proliferating pilar tumors arising in KID syndrome: a report of two patients. Am J Med Genet A 143:734-41

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