Abstract

This core will consist of a Protein Chemistry facility under Dr. Lu, an Analytical Ultracentrifugation facility under Walter. F. Stafford, Ph.D., and an Electron Microscopy and Immunocytochemistry facility under Katsuhide Mabuchi, Ph.D. The Protein Chemistry part of the Core will provide services for all projects in making synthetic peptides and analyzing peptides/proteins for amino acid compositions and/or sequences. It will also provide advice concerning peptides/proteins purification and characterization. The Analytical Ultracentrifugation part of the Core will primarily support Projects 1, 2-A, 2-B and 3. This service provides both sedimentation velocity and sedimentation equilibrium analysis, as well as related hydrodynamic analyses. The third part of Core B will provide services with the combined use of electron microscopy and immunocytochemistry. It offers technical expertise and advice for the EM visualization of proteins, especially with an improved rotary shadowing technique developed by the PI, and supports the preparation of polyclonal and monoclonal antibodies, including the handling of laboratory animals.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Program Projects (P01)
Project #
3P01AR041637-10S1
Application #
6571150
Study Section
Project Start
2001-12-01
Project End
2002-11-30
Budget Start
Budget End
Support Year
10
Fiscal Year
2002
Total Cost
$276,287
Indirect Cost

  Institution

Name
Boston Biomedical Research Institute
Department
Type
DUNS #
058893371
City
Watertown
State
MA
Country
United States
Zip Code
02472

  Publications

Guo, Hongqiu; Huang, Renjian; Semba, Shingo et al. (2013) Ablation of smooth muscle caldesmon affects the relaxation kinetics of arterial muscle. Pflugers Arch 465:283-94
Mabuchi, Yasuko; Mabuchi, Katsuhide; Stafford, Walter F et al. (2010) Modular structure of smooth muscle Myosin light chain kinase: hydrodynamic modeling and functional implications. Biochemistry 49:2903-17
Gali?ska, Agnieszka; Hatch, Victoria; Craig, Roger et al. (2010) The C terminus of cardiac troponin I stabilizes the Ca2+-activated state of tropomyosin on actin filaments. Circ Res 106:705-11
Hayes, David; Napoli, Vanessa; Mazurkie, Andrew et al. (2009) Phosphorylation dependence of hsp27 multimeric size and molecular chaperone function. J Biol Chem 284:18801-7
Mudalige, Wasana A K A; Tao, Terence C; Lehrer, Sherwin S (2009) Ca2+-dependent photocrosslinking of tropomyosin residue 146 to residues 157-163 in the C-terminal domain of troponin I in reconstituted skeletal muscle thin filaments. J Mol Biol 389:575-83
Greenberg, M J; Wang, C-L A; Lehman, W et al. (2008) Modulation of actin mechanics by caldesmon and tropomyosin. Cell Motil Cytoskeleton 65:156-64
Coulton, Arthur T; Koka, Kezia; Lehrer, Sherwin S et al. (2008) Role of the head-to-tail overlap region in smooth and skeletal muscle beta-tropomyosin. Biochemistry 47:388-97
Sumida, John P; Wu, Eleanor; Lehrer, Sherwin S (2008) Conserved Asp-137 imparts flexibility to tropomyosin and affects function. J Biol Chem 283:6728-34
Wang, C L Albert (2008) Caldesmon and the regulation of cytoskeletal functions. Adv Exp Med Biol 644:250-72
Lee, Eunhee; Hayes, David B; Langsetmo, Knut et al. (2007) Interactions between the leucine-zipper motif of cGMP-dependent protein kinase and the C-terminal region of the targeting subunit of myosin light chain phosphatase. J Mol Biol 373:1198-212

Showing the most recent 10 out of 95 publications