Abstract

The Baculovirus Expression System will be used for expression of recombinant proteins in eukaryotic cells This eukaryotic expression system encompasses many of the post-translational modification pathways, allowing the production of recombinant protein which is antigenically, immunogenically, and functionally similar to the native protein (e.g. MLCK, MAPK). Also, the majority of this overproduced protein remains soluble in the insect cells and the viral genome is relatively large (130 kbp) and thus can accommodate large segments of foreign DNA ( e.g. myosin heavy chain, CaD, etc.). The dual-promoter co-expression vector (e.g. p2Bac) permits simultaneous expression of two recombinant proteins (e.g., myosin heavy chain and light chains). This core will serve Projects 1, 2-A and 2-C.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Program Projects (P01)
Project #
3P01AR041637-10S1
Application #
6571151
Study Section
Project Start
2001-12-01
Project End
2002-11-30
Budget Start
Budget End
Support Year
10
Fiscal Year
2002
Total Cost
$276,287
Indirect Cost

  Institution

Name
Boston Biomedical Research Institute
Department
Type
DUNS #
058893371
City
Watertown
State
MA
Country
United States
Zip Code
02472

  Publications

Guo, Hongqiu; Huang, Renjian; Semba, Shingo et al. (2013) Ablation of smooth muscle caldesmon affects the relaxation kinetics of arterial muscle. Pflugers Arch 465:283-94
Mabuchi, Yasuko; Mabuchi, Katsuhide; Stafford, Walter F et al. (2010) Modular structure of smooth muscle Myosin light chain kinase: hydrodynamic modeling and functional implications. Biochemistry 49:2903-17
Gali?ska, Agnieszka; Hatch, Victoria; Craig, Roger et al. (2010) The C terminus of cardiac troponin I stabilizes the Ca2+-activated state of tropomyosin on actin filaments. Circ Res 106:705-11
Hayes, David; Napoli, Vanessa; Mazurkie, Andrew et al. (2009) Phosphorylation dependence of hsp27 multimeric size and molecular chaperone function. J Biol Chem 284:18801-7
Mudalige, Wasana A K A; Tao, Terence C; Lehrer, Sherwin S (2009) Ca2+-dependent photocrosslinking of tropomyosin residue 146 to residues 157-163 in the C-terminal domain of troponin I in reconstituted skeletal muscle thin filaments. J Mol Biol 389:575-83
Greenberg, M J; Wang, C-L A; Lehman, W et al. (2008) Modulation of actin mechanics by caldesmon and tropomyosin. Cell Motil Cytoskeleton 65:156-64
Coulton, Arthur T; Koka, Kezia; Lehrer, Sherwin S et al. (2008) Role of the head-to-tail overlap region in smooth and skeletal muscle beta-tropomyosin. Biochemistry 47:388-97
Sumida, John P; Wu, Eleanor; Lehrer, Sherwin S (2008) Conserved Asp-137 imparts flexibility to tropomyosin and affects function. J Biol Chem 283:6728-34
Wang, C L Albert (2008) Caldesmon and the regulation of cytoskeletal functions. Adv Exp Med Biol 644:250-72
Lee, Eunhee; Hayes, David B; Langsetmo, Knut et al. (2007) Interactions between the leucine-zipper motif of cGMP-dependent protein kinase and the C-terminal region of the targeting subunit of myosin light chain phosphatase. J Mol Biol 373:1198-212

Showing the most recent 10 out of 95 publications