Core - Tissue Culture

Stein, Gary S.

Abstract

The Tissue Culture Core will support each of the projects by providing cells, technical support and facilities that include: 1. Routine isolation of primary fetal rat calvarial-derived and human derived osteoblasts as needed; 2. Maintenance of the cultures under defined experimental conditions throughout the osteoblast developmental period; 3. Maintenance of osteosarcoma and non-osseous cell lines; 4. Monitoring growth and differentiation parameters that include photography and histochemical detection procedures, cell counts, H- thymidine labelling; 5. Harvesting media and cell layers samples for assays by the Biochemistry and Molecular Biology Cores for specific experiments; 6. Preparation and sterility testing of cell culture media, screening of serum lots and reagents for the culture to ensure uniformity of cultures; 7. Collating parameters of cell growth-differentiation and tissue organization for distribution to project investigations.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
• Type: Research Program Projects (P01)
• Project #: 5P01AR042262-03
• Application #: 5206311
• Support Year: 3
• Fiscal Year: 1996

Publications

Shopland, L S; Byron, M; Stein, J L et al. (2001) Replication-dependent histone gene expression is related to Cajal body (CB) association but does not require sustained CB contact. Mol Biol Cell 12:565-76

Lian, J B; Stein, G S; Stein, J L et al. (1999) Regulated expression of the bone-specific osteocalcin gene by vitamins and hormones. Vitam Horm 55:443-509

Stein, G S; van Wijnen, A J; Stein, J L et al. (1999) Implications for interrelationships between nuclear architecture and control of gene expression under microgravity conditions. FASEB J 13 Suppl:S157-66

Stein, G S; van Wijnen, A J; Stein, J L et al. (1998) Linkages of nuclear architecture to biological and pathological control of gene expression. J Cell Biochem Suppl 30-31:220-31

Stein, G S; van Wijnen, A J; Stein, J L et al. (1998) Interrelationships of nuclear structure and transcriptional control: functional consequences of being in the right place at the right time. J Cell Biochem 70:200-12

Choi, J Y; van Wijnen, A J; Aslam, F et al. (1998) Developmental association of the beta-galactoside-binding protein galectin-1 with the nuclear matrix of rat calvarial osteoblasts. J Cell Sci 111 ( Pt 20):3035-43

Stein, G S; van Wijnen, A J; Stein, J L et al. (1998) Nuclear structure--skeletal gene expression interrelationships. Front Biosci 3:d849-64

McNeil, S; Guo, B; Stein, J L et al. (1998) Targeting of the YY1 transcription factor to the nucleolus and the nuclear matrix in situ: the C-terminus is a principal determinant for nuclear trafficking. J Cell Biochem 68:500-10

Ji, C; Casinghino, S; Chang, D J et al. (1998) CBFa(AML/PEBP2)-related elements in the TGF-beta type I receptor promoter and expression with osteoblast differentiation. J Cell Biochem 69:353-63

Lynch, M P; Capparelli, C; Stein, J L et al. (1998) Apoptosis during bone-like tissue development in vitro. J Cell Biochem 68:31-49