Core - Tissue Culture

Stein, Gary S.

Abstract

The Tissue Culture Core will support each of the projects by providing cells, technical support and facilities that include: 1. Routine isolation of primary fetal rat calvarial-derived and human derived osteoblasts as needed; 2. Maintenance of the cultures under defined experimental conditions throughout the osteoblast developmental period; 3. Maintenance of osteosarcoma and non-osseous cell lines; 4. Monitoring growth and differentiation parameters that include photography and histochemical detection procedures, cell counts, H- thymidine labelling; 5. Harvesting media and cell layers samples for assays by the Biochemistry and Molecular Biology Cores for specific experiments; 6. Preparation and sterility testing of cell culture media, screening of serum lots and reagents for the culture to ensure uniformity of cultures; 7. Collating parameters of cell growth-differentiation and tissue organization for distribution to project investigations.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
• Type: Research Program Projects (P01)
• Project #: 5P01AR042262-03
• Application #: 5206311
• Support Year: 3
• Fiscal Year: 1996

Publications

Shopland, L S; Byron, M; Stein, J L et al. (2001) Replication-dependent histone gene expression is related to Cajal body (CB) association but does not require sustained CB contact. Mol Biol Cell 12:565-76

Lian, J B; Stein, G S; Stein, J L et al. (1999) Regulated expression of the bone-specific osteocalcin gene by vitamins and hormones. Vitam Horm 55:443-509

Stein, G S; van Wijnen, A J; Stein, J L et al. (1999) Implications for interrelationships between nuclear architecture and control of gene expression under microgravity conditions. FASEB J 13 Suppl:S157-66

Lynch, M P; Capparelli, C; Stein, J L et al. (1998) Apoptosis during bone-like tissue development in vitro. J Cell Biochem 68:31-49

Zeng, C; McNeil, S; Pockwinse, S et al. (1998) Intranuclear targeting of AML/CBFalpha regulatory factors to nuclear matrix-associated transcriptional domains. Proc Natl Acad Sci U S A 95:1585-9

Shalhoub, V; Aslam, F; Breen, E et al. (1998) Multiple levels of steroid hormone-dependent control of osteocalcin during osteoblast differentiation: glucocorticoid regulation of basal and vitamin D stimulated gene expression. J Cell Biochem 69:154-68

Lindenmuth, D; van Wijnen, A J; Penman, S et al. (1998) TGF-beta1 modifications in nuclear matrix proteins of osteoblasts during differentiation. J Cell Biochem 69:291-303

Bagchi, M; Ansari, S A; Lindenmuth, D M et al. (1998) Nuclear matrix associated DNA-binding proteins of ocular lens epithelial cells. Mol Biol Rep 25:13-9

Stein, G S; van Wijnen, A J; Stein, J L et al. (1998) Linkages of nuclear architecture to biological and pathological control of gene expression. J Cell Biochem Suppl 30-31:220-31

Stein, G S; van Wijnen, A J; Stein, J L et al. (1998) Interrelationships of nuclear structure and transcriptional control: functional consequences of being in the right place at the right time. J Cell Biochem 70:200-12