Core - Molecular Biology

Stein, Janet L.

Abstract

The Molecular Biology Core will support the various projects in this program by providing the following reagents and services: 1. Isolation and maintenance of shared pools of characterized osteoblast and osteosarcoma RNAs; northern blot analysis and nuclear run-on analysis as necessary. 2. Oligonucleotide synthesis. 3. Preparation and labeling of probes used routinely or in common by the projects. 4. Growth of recombinant DNA plasmids and phage. 5. Maintenance and permanent storage of plasmid, bacterial, and phage stocks (including bacterial hosts, vectors, libraries, etc.). 6. Preparation of plates and media for bacterial culture. 7. Construction of cDNA libraries. 8. Assistance with special projects, such as screening of cDNA and genomic libraries, or large DNA sequencing projects.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
• Type: Research Program Projects (P01)
• Project #: 5P01AR042262-03
• Application #: 5206312
• Support Year: 3
• Fiscal Year: 1996

Publications

Shopland, L S; Byron, M; Stein, J L et al. (2001) Replication-dependent histone gene expression is related to Cajal body (CB) association but does not require sustained CB contact. Mol Biol Cell 12:565-76

Lian, J B; Stein, G S; Stein, J L et al. (1999) Regulated expression of the bone-specific osteocalcin gene by vitamins and hormones. Vitam Horm 55:443-509

Stein, G S; van Wijnen, A J; Stein, J L et al. (1999) Implications for interrelationships between nuclear architecture and control of gene expression under microgravity conditions. FASEB J 13 Suppl:S157-66

McNeil, S; Guo, B; Stein, J L et al. (1998) Targeting of the YY1 transcription factor to the nucleolus and the nuclear matrix in situ: the C-terminus is a principal determinant for nuclear trafficking. J Cell Biochem 68:500-10

Ji, C; Casinghino, S; Chang, D J et al. (1998) CBFa(AML/PEBP2)-related elements in the TGF-beta type I receptor promoter and expression with osteoblast differentiation. J Cell Biochem 69:353-63

Lynch, M P; Capparelli, C; Stein, J L et al. (1998) Apoptosis during bone-like tissue development in vitro. J Cell Biochem 68:31-49

Zeng, C; McNeil, S; Pockwinse, S et al. (1998) Intranuclear targeting of AML/CBFalpha regulatory factors to nuclear matrix-associated transcriptional domains. Proc Natl Acad Sci U S A 95:1585-9

Shalhoub, V; Aslam, F; Breen, E et al. (1998) Multiple levels of steroid hormone-dependent control of osteocalcin during osteoblast differentiation: glucocorticoid regulation of basal and vitamin D stimulated gene expression. J Cell Biochem 69:154-68

Lindenmuth, D; van Wijnen, A J; Penman, S et al. (1998) TGF-beta1 modifications in nuclear matrix proteins of osteoblasts during differentiation. J Cell Biochem 69:291-303

Bagchi, M; Ansari, S A; Lindenmuth, D M et al. (1998) Nuclear matrix associated DNA-binding proteins of ocular lens epithelial cells. Mol Biol Rep 25:13-9