Several projects in this application require the generation of genetically modified mouse embryonic stem (ES) cell lines. Gene targeting experiments are technically challenging and time-consuming even under the best circumstances. This is particularly true for laboratories that do not perform these experiments on a regular basis. One major concern is ES cell cultures. Although ES cells might appear morphologically normal, they might lose their ability to contribute to germline transmission of a mutation due to sub-optimal culture conditions. It can take several months to identify ES cell clones that do not contribute to germline transmission. It is therefore desirable to conduct these experiments in a central facility with experienced personnel. This will ensure that these experiments are done in the most efficient manner with respect to costs, time and quality. The ES Core will provide expertise in the design of targeting vectors and will perform all ES cell manipulations. This serve will include the generation and amplification of targeted ES cell clones and the preparation of cells for blastocyst injection (which will be done in Core B, Director, Franco DeMayo). The principled investigators will supply the targeting construct and will be responsible for screening the ES cell DNA for correct targeting.
| Hoelzl, Maria A; Heby-Henricson, Karin; Gerling, Marco et al. (2017) Differential requirement of SUFU in tissue development discovered in a hypomorphic mouse model. Dev Biol 429:132-146 |
| Rice, Robert H; Durbin-Johnson, Blythe P; Ishitsuka, Yosuke et al. (2016) Proteomic Analysis of Loricrin Knockout Mouse Epidermis. J Proteome Res 15:2560-6 |
| Ishitsuka, Yosuke; Huebner, Aaron J; Rice, Robert H et al. (2016) Lce1 Family Members Are Nrf2-Target Genes that Are Induced to Compensate for the Loss of Loricrin. J Invest Dermatol 136:1656-1663 |
| Zhang, Cong; Large, Michael J; Duggavathi, Raj et al. (2013) Liver receptor homolog-1 is essential for pregnancy. Nat Med 19:1061-6 |
| Fiaschi, Marie; Kolterud, Asa; Nilsson, Mats et al. (2011) Targeted expression of GLI1 in the salivary glands results in an altered differentiation program and hyperplasia. Am J Pathol 179:2569-79 |
| Dai, Daisy; Zhu, Huiping; Wlodarczyk, Bogdan et al. (2011) Fuz controls the morphogenesis and differentiation of hair follicles through the formation of primary cilia. J Invest Dermatol 131:302-10 |
| Honeycutt, Kimberly A; Waikel, Rebekah L; Koster, Maranke I et al. (2010) The effect of c-myc on stem cell fate influences skin tumor phenotype. Mol Carcinog 49:315-9 |
| Jaks, Viljar; Kasper, Maria; Toftgard, Rune (2010) The hair follicle-a stem cell zoo. Exp Cell Res 316:1422-8 |
| Kim, Soeun; Choi, Irene F; Quante, Jessica R et al. (2009) p63 directly induces expression of Alox12, a regulator of epidermal barrier formation. Exp Dermatol 18:1016-21 |
| Koster, Maranke I; Marinari, Barbara; Payne, Aimee S et al. (2009) DeltaNp63 knockdown mice: A mouse model for AEC syndrome. Am J Med Genet A 149A:1942-7 |
Showing the most recent 10 out of 53 publications
