Abstract

This Core will provide advice, vectors, and as needed actual vector construction and validation for the production of needed knockin and BAC Tg mice. It will also provide initial validation and breeding of such mice, and will transfer mice as needed to the individual projects. However, it will not generally be a mouse- breeding repository. Finally, the Core will provide access to and advice on mouse SNP genotyping which will allow the projects to appropriately conduct """"""""speed-congenic"""""""" type backcrossing programs. This in turn will make new strains ready for use much more quickly, thus improving the efficiency ofthe projects overall. It will also provide important quality control over the genetic content ofthe strains used in the project.

Public Health Relevance

This Core will provide important support for genetic experiments that will help elucidate how lupus develops in live animals, which is the only good model for human lupus.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Program Projects (P01)
Project #
5P01AR050256-08
Application #
8120848
Study Section
Special Emphasis Panel (ZAR1)
Project Start
2010-07-01
Project End
2014-06-30
Budget Start
2010-07-01
Budget End
2011-06-30
Support Year
8
Fiscal Year
2010
Total Cost
$2
Indirect Cost

  Institution

Name
University of Massachusetts Medical School Worcester
Department
Type
DUNS #
603847393
City
Worcester
State
MA
Country
United States
Zip Code
01655

  Publications

Pawaria, Sudesh; Sharma, Shruti; Baum, Rebecca et al. (2017) Taking the STING out of TLR-driven autoimmune diseases: good, bad, or indifferent? J Leukoc Biol 101:121-126
Sindhava, Vishal J; Oropallo, Michael A; Moody, Krishna et al. (2017) A TLR9-dependent checkpoint governs B cell responses to DNA-containing antigens. J Clin Invest 127:1651-1663
Baum, Rebecca; Sharma, Shruti; Organ, Jason M et al. (2017) STING Contributes to Abnormal Bone Formation Induced by Deficiency of DNase II in Mice. Arthritis Rheumatol 69:460-471
Roberts, Allison W; Lee, Bettina L; Deguine, Jacques et al. (2017) Tissue-Resident Macrophages Are Locally Programmed for Silent Clearance of Apoptotic Cells. Immunity 47:913-927.e6
Giles, Josephine R; Neves, Adriana Turqueti; Marshak-Rothstein, Ann et al. (2017) Autoreactive helper T cells alleviate the need for intrinsic TLR signaling in autoreactive B cell activation. JCI Insight 2:e90870
Bossaller, Lukas; Christ, Anette; Pelka, Karin et al. (2016) TLR9 Deficiency Leads to Accelerated Renal Disease and Myeloid Lineage Abnormalities in Pristane-Induced Murine Lupus. J Immunol 197:1044-53
Garcia-Martinez, Irma; Santoro, Nicola; Chen, Yonglin et al. (2016) Hepatocyte mitochondrial DNA drives nonalcoholic steatohepatitis by activation of TLR9. J Clin Invest 126:859-64
Baum, Rebecca; Nündel, Kerstin; Pawaria, Sudesh et al. (2016) Synergy between Hematopoietic and Radioresistant Stromal Cells Is Required for Autoimmune Manifestations of DNase II-/-IFNaR-/- Mice. J Immunol 196:1348-54
Moody, Krishna L; Uccellini, Melissa B; Avalos, Ana M et al. (2016) Toll-Like Receptor-Dependent Immune Complex Activation of B Cells and Dendritic Cells. Methods Mol Biol 1390:249-72
Pawaria, Sudesh; Moody, Krishna L; Busto, Patricia et al. (2015) An unexpected role for RNA-sensing toll-like receptors in a murine model of DNA accrual. Clin Exp Rheumatol 33:S70-3

Showing the most recent 10 out of 65 publications