Abstract

Dr. Ames will be directing the biochemistry core at CHORI which will carry out various assays in serum, plasma and airway fluid samples collected in the conduct of all three projects. Dr. Ames laboratory has extensive experience in bioassays and have developed numerous assays for biomarkers for oxidative damage and antioxidants. These assays include HPLC and GC-MS based detection of antioxidants and oxidized products in biological samples. The assays include detection of tocopherol, 5-Nitro-gamma-tocopherol (a stable adduct formed by oxidation of gamma-tocopherol), beta-CEHC (2,7,8-trimethyl-2-(beta-carboxyethyl)-6-hydroxychroman, a metabolite. They have also developed assays to assess levels of 5-Chlorouracil (5-CIU) and 5-Bromouracil (5-BrU), markers for the action of myeloperoxidase (MPO) and eosinophil peroxidase (EPO) from granulocytes involved in airway inflammation. The assays, mostly developed in the Ames lab, for detection of oxidative damage include lipid peroxidation (MDA assay, a GC-MS method), and DNA damage markers: AP sites, and comet assays, 8-oxo-Guanine, DNA-protein cross links, and double-strand breaks (micronuclei).

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Complementary & Alternative Medicine (NCCAM)
Type
Research Program Projects (P01)
Project #
1P01AT002620-01
Application #
6883688
Study Section
Special Emphasis Panel (ZAT1-CP (15))
Project Start
2004-11-17
Project End
2009-06-30
Budget Start
2004-11-17
Budget End
2005-06-30
Support Year
1
Fiscal Year
2004
Total Cost
$127,139
Indirect Cost

  Institution

Name
University of North Carolina Chapel Hill
Department
Type
DUNS #
608195277
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599

  Publications

Wagner, James G; Birmingham, Neil P; Jackson-Humbles, Daven et al. (2014) Supplementation with ?-tocopherol attenuates endotoxin-induced airway neutrophil and mucous cell responses in rats. Free Radic Biol Med 68:101-9
Mills, K; Lay, J; Wu, W et al. (2014) Vitamin E, ?-tocopherol, diminishes ex vivo basophil response to dust mite allergen. Allergy 69:541-4
Fry, Rebecca C; Rager, Julia E; Bauer, Rebecca et al. (2014) Air toxics and epigenetic effects: ozone altered microRNAs in the sputum of human subjects. Am J Physiol Lung Cell Mol Physiol 306:L1129-37
Geiser, Marianne; Lay, John C; Bennett, William D et al. (2013) Effects of ex vivo ?-tocopherol on airway macrophage function in healthy and mild allergic asthmatics. J Innate Immun 5:613-24
Hernandez, Michelle L; Wagner, James G; Kala, Aline et al. (2013) Vitamin E, ?-tocopherol, reduces airway neutrophil recruitment after inhaled endotoxin challenge in rats and in healthy volunteers. Free Radic Biol Med 60:56-62
Fry, Rebecca C; Rager, Julia E; Zhou, Haibo et al. (2012) Individuals with increased inflammatory response to ozone demonstrate muted signaling of immune cell trafficking pathways. Respir Res 13:89
Wang, Yun; Moreland, Michelle; Wagner, James G et al. (2012) Vitamin E forms inhibit IL-13/STAT6-induced eotaxin-3 secretion by up-regulation of PAR4, an endogenous inhibitor of atypical PKC in human lung epithelial cells. J Nutr Biochem 23:602-8
Lay, John C; Peden, David B; Alexis, Neil E (2011) Flow cytometry of sputum: assessing inflammation and immune response elements in the bronchial airways. Inhal Toxicol 23:392-406
Esther Jr, Charles R; Peden, David B; Alexis, Neil E et al. (2011) Airway purinergic responses in healthy, atopic nonasthmatic, and atopic asthmatic subjects exposed to ozone. Inhal Toxicol 23:324-30
Dillon, Madeline A; Harris, Bradford; Hernandez, Michelle L et al. (2011) Enhancement of systemic and sputum granulocyte response to inhaled endotoxin in people with the GSTM1 null genotype. Occup Environ Med 68:783-5

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