The Analytical Core will serve as a centralized facility aimed at assisting investigators to pursue high quality research on all the projects of the program. It will also foster synergistic research by determining the epigenetic mechanisms involved in regulation of inflammatory diseases following treatment with botanicals.
The specific aims of the Core are 1) to provide state-of-the-art tools and technology as well as training for pursuing epigenomics studies including data acquisition and high throughput analysis of DNA methylation, histone methylation and acetylation as well as microRNA. Determination of epigenomic changes can lead to development of preventative and therapeutic strategies as well as identification of biomarkers and molecular signatures for diagnosis and prognosis. The study of epigenomics is an emerging area of research and necessitates the use of novel tools and technology made possible by the core personnel with expertise to perform genome-wide analysis without duplication of resources. 2) To utilize statistical approaches in developing experimental design and evaluation of the significance of the data obtained in all the projects as well as to use bioinformatic approaches including data mining, sharing and archiving of big datasets. This would involve development of experimental protocols, troubleshooting, statistical evaluation of the data generated and interpretation. The Core will also coordinate data collection, analysis, and accessibility of the data collected by the investigators. The Core will use bioinformatic approaches for data-mining, processing and archiving of data sets obtained through high-throughput analysis. This would include data extraction, warehousing and visualization. It will use computational data analysis using databases such as gene ontology and pathway analysis. 3) To conduct standard toxicity testing and immune monitoring of all the plant-derived compounds used in the 4 projects by performing screening for systemic toxicity as well as the 2-tier Immunotoxicity testing. While mechanistic studies in projects are restricted primarily to specific immune cells, it is critical to know how such plant-derived components affect the overall immune system. This is important because autoimmune and inflammatory diseases are often systemic and involve multiple types of immune cells. The toxicity studies are also useful because the data generated are pivotal for developing future translational research. 4) To perform evaluation of the natural product quality by performing chemical analysis thereby ensuring accurate identification and quantification of the components including potential impurities, quality control of batches of herbal product used, and stability following storage. In summary, the core will conduct epigenomic studies, statistical evaluation, bioinformatic approaches of data analysis, toxicity testing, immune monitoring, and natural product integrity of botanicals that would benefit all the projects of the PPG.

National Institute of Health (NIH)
National Center for Complementary & Alternative Medicine (NCCAM)
Research Program Projects (P01)
Project #
Application #
Study Section
Special Emphasis Panel (ZAT1)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of South Carolina at Columbia
United States
Zip Code
Wirth, Michael D; Sevoyan, Maria; Hofseth, Lorne et al. (2018) The Dietary Inflammatory Index is associated with elevated white blood cell counts in the National Health and Nutrition Examination Survey. Brain Behav Immun 69:296-303
Becker, William; Nagarkatti, Mitzi; Nagarkatti, Prakash S (2018) miR-466a Targeting of TGF-?2 Contributes to FoxP3+ Regulatory T Cell Differentiation in a Murine Model of Allogeneic Transplantation. Front Immunol 9:688
Dopkins, Nicholas; Nagarkatti, Prakash S; Nagarkatti, Mitzi (2018) The role of gut microbiome and associated metabolome in the regulation of neuroinflammation in multiple sclerosis and its implications in attenuating chronic inflammation in other inflammatory and autoimmune disorders. Immunology 154:178-185
Abron, Jessica D; Singh, Narendra P; Mishra, Manoj K et al. (2018) An endogenous aryl hydrocarbon receptor ligand, ITE, induces regulatory T cells and ameliorates experimental colitis. Am J Physiol Gastrointest Liver Physiol 315:G220-G230
Alghetaa, Hasan; Mohammed, Amira; Sultan, Muthanna et al. (2018) Resveratrol protects mice against SEB-induced acute lung injury and mortality by miR-193a modulation that targets TGF-? signalling. J Cell Mol Med 22:2644-2655
Zhang, Tao; Zhou, Juhua; Man, Gene Chi Wai et al. (2018) MDSCs drive the process of endometriosis by enhancing angiogenesis and are a new potential therapeutic target. Eur J Immunol 48:1059-1073
Seth, Ratanesh Kumar; Kimono, Diana; Alhasson, Firas et al. (2018) Increased butyrate priming in the gut stalls microbiome associated-gastrointestinal inflammation and hepatic metabolic reprogramming in a mouse model of Gulf War Illness. Toxicol Appl Pharmacol 350:64-77
Pageni, Parasmani; Yang, Peng; Chen, Yung Pin et al. (2018) Charged Metallopolymer-Grafted Silica Nanoparticles for Antimicrobial Applications. Biomacromolecules 19:417-425
Xin, Ying; Bai, Yang; Jiang, Xin et al. (2018) Sulforaphane prevents angiotensin II-induced cardiomyopathy by activation of Nrf2 via stimulating the Akt/GSK-3ß/Fyn pathway. Redox Biol 15:405-417
Dubey, Seema; Yoon, Hyunho; Cohen, Mark Steven et al. (2018) Withaferin A Associated Differential Regulation of Inflammatory Cytokines. Front Immunol 9:195

Showing the most recent 10 out of 178 publications