Abstract

Elucidation of the biology of initiation and emergence of epithelial neoplasms, as illustrated by mammary and cervicovaginal tumorigenesis, is the overall objective of this proposal. Our research plan combines a variety of in vivo and in vitro experimental approaches to these problems, utilizing mouse and rat model systems and, in some cases, human cells. Experiments are designed to analyzed the cellular composition of the mammary gland and its tumors and to examine the roles of hormones, viruses, chemical agents, and cellular and host factors in the growth of normal and neoplastic cells, including metastatic tumors. Cervicovaginal studies with the mouse model will focus on dysplasias induced by perinatal steroid administration and their subsequent transformation into carcinomas in host environments modified by hormonal and immunological manipulations. Antigenicity and the metastatic behavior of these carcinomas will also be analyzed. We expect that our studies on these two systems will generate models that can be used for both in vitro and in vivo analyses of other epithelial neoplasms.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA005388-27
Application #
3092481
Study Section
Cancer Special Program Advisory Committee (CAK)
Project Start
1980-05-01
Project End
1990-04-30
Budget Start
1986-05-01
Budget End
1987-04-30
Support Year
27
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of California Berkeley
Department
Type
Schools of Arts and Sciences
DUNS #
094878337
City
Berkeley
State
CA
Country
United States
Zip Code
94704

  Publications

Bera, T K; Guzman, R C; Miyamoto, S et al. (1994) Identification of a mammary transforming gene (MAT1) associated with mouse mammary carcinogenesis. Proc Natl Acad Sci U S A 91:9789-93
Bera, T K; Hwang, S I; Swanson, S M et al. (1994) In situ localization of prolactin receptor message in the mammary glands of pituitary-isografted mice. Mol Cell Biochem 132:145-9
Swanson, S M; Guzman, R C; Christov, K et al. (1994) Pituitary-isografted mice are highly susceptible to MNU-induced mammary carcinogenesis irrespective of the level of alveolar differentiation. Carcinogenesis 15:1341-6
McKenzie, K E; Bandyopadhyay, G K; Imagawa, W et al. (1994) Omega-3 and omega-6 fatty acids and PGE2 stimulate the growth of normal but not tumor mouse mammary epithelial cells: evidence for alterations in the signaling pathways in tumor cells. Prostaglandins Leukot Essent Fatty Acids 51:437-43
Pavlova, A; Boutin, E; Cunha, G et al. (1994) Msx1 (Hox-7.1) in the adult mouse uterus: cellular interactions underlying regulation of expression. Development 120:335-45
Cunha, G R (1994) Role of mesenchymal-epithelial interactions in normal and abnormal development of the mammary gland and prostate. Cancer 74:1030-44
Goya, L; Maiyar, A C; Ge, Y et al. (1993) Glucocorticoids induce a G1/G0 cell cycle arrest of Con8 rat mammary tumor cells that is synchronously reversed by steroid withdrawal or addition of transforming growth factor-alpha. Mol Endocrinol 7:1121-32
Webster, M K; Goya, L; Ge, Y et al. (1993) Characterization of sgk, a novel member of the serine/threonine protein kinase gene family which is transcriptionally induced by glucocorticoids and serum. Mol Cell Biol 13:2031-40
Webster, M K; Goya, L; Firestone, G L (1993) Immediate-early transcriptional regulation and rapid mRNA turnover of a putative serine/threonine protein kinase. J Biol Chem 268:11482-5
Iguchi, T; Edery, M; Tasi, P S et al. (1993) Epidermal growth factor receptor levels in reproductive organs of female mice exposed neonatally to diethylstilbestrol. Proc Soc Exp Biol Med 204:110-6

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