The long-term objective of the Scientific Core is to provide state-of-the-art Core facilities that support each of the four projects of the Program. Core B is composed of a Phosphorimager facility and a protein expression and purification facility, as well as other shared facilities including a dark room and liquid nitrogen freezers. The Phosphorimager will be used for imaging and quantification of various results including primer-extension assays, gel mobility shifts, and Western blots. The protein expression and purification facility consists of a shaking incubator, a centrifuge, an FPLC, and protein expression vectors. It is used to express and purify proteins that are studied in each of the projects. The Scientific Core also possesses liquid nitrogen freezers for the storage of cell lines and a gel documentation system. In addition, expertise is provided to all member laboratories by personnel who oversee the Core. This Scientific Core is essential to the success of the Program, and serves to facilitate interaction between member laboratories.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA016038-35
Application #
7669335
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2008-07-01
Budget End
2009-06-30
Support Year
35
Fiscal Year
2008
Total Cost
$100,762
Indirect Cost
Name
Yale University
Department
Type
DUNS #
043207562
City
New Haven
State
CT
Country
United States
Zip Code
06520
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