Abstract

The long-term goal of the proposed research is to understand the molecular mechanisms involved in the replication of chromosomes in eukaryotic cells, particularly the mechanisms that regulate DNA replication during the cell cycle. This goal is a fundamental aspect of the more general objectives of the Program to understand the basic mechanisms that control cell growth and maintain genomic stability. Our approach to this problem involves genetic and biochemical studies of the initiation of DNA replication in the model organism Schizosaccharomyces pombe. We plan to focus our efforts in two general areas. First, we will continue our biochemical studies to identify, purify and characterize initiator proteins and to define their interactions with origins of DNA replication. Second, we will study how the initiation of DNA replication is regulated by the specific phosphorylation of cellular initiation proteins.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
3P01CA016519-26S1
Application #
6417624
Study Section
Project Start
2001-02-07
Project End
2001-03-31
Budget Start
Budget End
Support Year
26
Fiscal Year
2001
Total Cost
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Type
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Janes, K; Symons-Liguori, A M; Jacobson, K A et al. (2016) Identification of A3 adenosine receptor agonists as novel non-narcotic analgesics. Br J Pharmacol 173:1253-67
Oh, Sekyung; Kato, Masaki; Zhang, Chi et al. (2015) A Comparison of Ci/Gli Activity as Regulated by Sufu in Drosophila and Mammalian Hedgehog Response. PLoS One 10:e0135804
Price, Jessica C; Pollock, Lana M; Rudd, Meghan L et al. (2014) Sequencing of candidate chromosome instability genes in endometrial cancers reveals somatic mutations in ESCO1, CHTF18, and MRE11A. PLoS One 8:e63313
O'Donnell, Kathryn A; An, Wenfeng; Schrum, Christina T et al. (2013) Controlled insertional mutagenesis using a LINE-1 (ORFeus) gene-trap mouse model. Proc Natl Acad Sci U S A 110:E2706-13
Newman, Robert H; Hu, Jianfei; Rho, Hee-Sool et al. (2013) Construction of human activity-based phosphorylation networks. Mol Syst Biol 9:655
Gnanakkan, Veena P; Jaffe, Andrew E; Dai, Lixin et al. (2013) TE-array--a high throughput tool to study transposon transcription. BMC Genomics 14:869
Rybanska-Spaeder, Ivana; Reynolds, Taylor L; Chou, Jeremy et al. (2013) 53BP1 is limiting for NHEJ repair in ATM-deficient model systems that are subjected to oncogenic stress or radiation. Mol Cancer Res 11:1223-34
Le Gallo, Matthieu; O'Hara, Andrea J; Rudd, Meghan L et al. (2012) Exome sequencing of serous endometrial tumors identifies recurrent somatic mutations in chromatin-remodeling and ubiquitin ligase complex genes. Nat Genet 44:1310-5
O'Donnell, Kathryn A; Keng, Vincent W; York, Brian et al. (2012) A Sleeping Beauty mutagenesis screen reveals a tumor suppressor role for Ncoa2/Src-2 in liver cancer. Proc Natl Acad Sci U S A 109:E1377-86
Burns, Kathleen H; Boeke, Jef D (2012) Human transposon tectonics. Cell 149:740-52

Showing the most recent 10 out of 246 publications