Core E: Cell Processing and Storage will support this grant proposal by providing four specific functions.First, this core will provide critical instrumentation and technical expertise in cell procurement and processingof all clinical components necessary to support the treatment of patients with malignant diseases describedin Projects 1-6. Direct funding is not requested for most of that activity, which is generally reimbursed bythird party payers, but Core E will also procure and distribute research specimens and provide additionalsummary data to investigators. Second, the Core will characterize the bone marrow and PBSC componentsutilized in these allogeneic transplantation studies by quantitative flow cytometry for various T-cell and otherlymphoid subsets. These data will be useful in determining which specific cell lineages may be correlatedwith clinical outcomes, which will in turn help to stimulate the development of novel graft engineeringapproaches and techniques that could improve future allogeneic transplantation studies. Third, Core E willoperate a Repository of Research Specimens. This Repository will consist of two distinct types ofcomponents: (1) aliquots of patient and normal donor blood and/or bone marrow samples; (2) DNApreparations and established B-LCL lines obtained from allogeneic transplant patients and theircorresponding normal donors. These samples will be critical in helping to define the mechanisms involved inengraftment, rejection, anti-tumor response and disease control, immune reconstitution, and thedevelopment of graft-versus-host disease. Material from this Repository will support studies outlined inProjects 1 , 2, 5, and 6. Fourth, Core E will be responsible for the development and clinical implementationof cell processing techniques necessary for the cGMP production of the WT1 and PR3 T-cells described inProject 5, and for the depletion of naive T-cells and subsequent generation of primed donor dendritic-cellsdescribed in Project 6. Integration of the clinical cell processing services of Core E with the projects containedwithin this grant application provides for better patient care, a higher degree of QA/QC, and significantlyreduced efforts and costs.Relevance to public health: The support of Core E is critical to the success of the work proposed in theindividual projects of this grant. This work promises to advance the field of allogeneic hematopoietictransplantation, making it safer, more effective, increasingly available and more appropriately applied. Suchan advance could have obvious relevance to the thousands of patients who are transplanted each year orare potential candidates for transplantation.
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