For years 12-17 of this program project we plan to continue to focus on mechanisms of latency and oncogenesis of the Epstein-Barr Virus, supplemented by studies of other viruses that produce latent infections, selected because they offer advantages for and reinforce the central investigational thrust. The main lines of research deal with EBV gene functions that may be important in producing cell transformation, mechanisms of episomal maintenance in latent EBV infection, regulation of EBV gene expression, especially those that may govern latency, oncogenic functions and virus reactivation.and interrelated studies of vitally encoded DNA polymerase with emphasis on expression and mutagenesis. This is a deliberately refashioned program project, taking advantage of new strengths and resources of the Lineberger Cancer Research Center and designed to be a cohesive interdisciplinary program. Participants who are PIs or Co-PIs are faculty members in six departments and interdisciplinary programs: Microbiology, Biochemistry, Medicine, Genetics, Molecular Biology and Cancer. New PIs in the program are: Dr. Nancy Raab-Traub, Dr. Shannon Kenney, and Dr. Ron swanstrom. The projects are as follows: Nancy Raab-Traub, Ph.D.: """"""""Oncogenic potential of EBV latency and transforming genes""""""""; Shannon Kenney, M.D.: """"""""Negative regulation of EBV gene expression""""""""; Jung_Chung Lin, Ph.D. and Joseph S. Pagano, M.D. : """"""""Epstein-Barr Virus DNS-binding proteins""""""""; Steven Bachenheimer, Ph.D.: """"""""DNS-protein interactions in HSV and EBV DNA polymerase: expression and regulation""""""""; Eng- Shang Huang, Ph.D.: """"""""Human cytomegalovirus-specific DNA polymerase""""""""; Ronald Swanstrom, Ph.D.: """"""""Hiv 2 and SIV pol genes"""""""". The core components consist of Tissue Culture, Electron Microscopy (Jack Griffith, Ph.D.) and Virus Containment. This assemblage of faculty and projects, building on the past accomplishments of the VPP, is expected to yield important new information and insights bearing on central issues of the molecular pathobiology of EBV.

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National Cancer Institute (NCI)
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University of North Carolina Chapel Hill
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DeKroon, Robert M; Gunawardena, Harsha P; Edwards, Rachel et al. (2018) Global Proteomic Changes Induced by the Epstein-Barr Virus Oncoproteins Latent Membrane Protein 1 and 2A. MBio 9:
Nicholls, Thomas J; Nadalutti, Cristina A; Motori, Elisa et al. (2018) Topoisomerase 3? Is Required for Decatenation and Segregation of Human mtDNA. Mol Cell 69:9-23.e6
El-Mallawany, Nader Kim; Kamiyango, William; Villiera, Jimmy et al. (2018) Proposal of a Risk-Stratification Platform to Address Distinct Clinical Features of Pediatric Kaposi Sarcoma in Lilongwe, Malawi. J Glob Oncol :1-7
Selitsky, Sara R; Marron, David; Mose, Lisle E et al. (2018) Epstein-Barr Virus-Positive Cancers Show Altered B-Cell Clonality. mSystems 3:
Hosseinipour, Mina C; Kang, Minhee; Krown, Susan E et al. (2018) As-Needed Vs Immediate Etoposide Chemotherapy in Combination With Antiretroviral Therapy for Mild-to-Moderate AIDS-Associated Kaposi Sarcoma in Resource-Limited Settings: A5264/AMC-067 Randomized Clinical Trial. Clin Infect Dis 67:251-260
Lyons, Danielle E; Yu, Kuan-Ping; Vander Heiden, Jason A et al. (2018) Mutant Cellular AP-1 Proteins Promote Expression of a Subset of Epstein-Barr Virus Late Genes in the Absence of Lytic Viral DNA Replication. J Virol 92:
Bigi, Rachele; Landis, Justin T; An, Hyowon et al. (2018) Epstein-Barr virus enhances genome maintenance of Kaposi sarcoma-associated herpesvirus. Proc Natl Acad Sci U S A 115:E11379-E11387
El-Mallawany, Nader Kim; Villiera, Jimmy; Kamiyango, William et al. (2018) Endemic Kaposi sarcoma in HIV-negative children and adolescents: an evaluation of overlapping and distinct clinical features in comparison with HIV-related disease. Infect Agent Cancer 13:33
Kobayashi, E; Aga, M; Kondo, S et al. (2018) C-Terminal Farnesylation of UCH-L1 Plays a Role in Transport of Epstein-Barr Virus Primary Oncoprotein LMP1 to Exosomes. mSphere 3:
Hopcraft, Sharon E; Pattenden, Samantha G; James, Lindsey I et al. (2018) Chromatin remodeling controls Kaposi's sarcoma-associated herpesvirus reactivation from latency. PLoS Pathog 14:e1007267

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